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Series GSE178647 Query DataSets for GSE178647
Status Public on Sep 24, 2021
Title ATAD2 maintains the equilibrium between nucleosome assembly and disassembly
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary To avoid toxic histone overload, histone deposition and removal must be finely tuned. Following a series of functional studies in human, mouse and S. pombe, we show here that the AAA ATPase ATAD2 is a conserved mediator of histone removal and a regulator of histone deposition. In Atad2 knockout (KO) spermatogenic cells, the late-stage genome-wide eviction of histones and their replacement by protamines are significantly delayed. In S. pombe, deletion of abo1 leads to a dramatic decrease in cell growth, with the appearance of suppressor clones recovering normal growth. Identification of the corresponding suppressor mutations revealed that the causes of cell toxicity are mainly the deregulated activity of the histone chaperone HIRA and histone overload. We also show that, in Atad2 KO mouse ES cells, HIRA and FACT are trapped on nucleosomes at transcription start sites of active genes, resulting in histone overload and abnormal histone deposition on nucleosome-free regions. Overall, these data shed new light on a need for energy-consuming auxiliary factors, such as ATAD2, to maintain a balanced and dynamic histone chaperone basal activity and to avoid a deleterious histone overload.
Overall design ChIP-seq experiments with HIRA and SSRP1 antibodies in wild type (WT) and ATAD2 KO (KO) embryonic stem (ES) cells
Contributor(s) Rousseaux S, Chuffart F, Wang T, Barral S, Puthier D, Bargier L, Khochbin S
Citation(s) 34580178
Submission date Jun 22, 2021
Last update date Sep 29, 2021
Contact name Sophie Rousseaux
Organization name INSERM-UGA
Department IAB
Lab U1209
Street address Bd de la Chantourne
City La Tronche
ZIP/Postal code 38706
Country France
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM5395228 Input WT rep2
GSM5395229 Input KO rep2
GSM5395230 SSRP1 WT rep2
BioProject PRJNA739885
SRA SRP325024

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Supplementary file Size Download File type/resource
GSE178647_RAW.tar 2.0 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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