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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 16, 2022 |
| Title |
Coordinated metabolic transitions and gene expression by NAD+ during adipogenesis. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Adipocytes are the main cell type in adipose tissue, a critical regulator of metabolism, highly specialized in storing energy as fat. Adipocytes differentiate from multipotent mesenchymal stromal cells through adipogenesis. Using RNA-seq we explored the effect of manipulating NAD+ bioavailability during adipogenic differentiation from human mesenchymal stem cells. Cells were harvested either undifferentiated (hMSC) or after days 8 and 16 of adipogenic induction. During adipogenesis, cells were treated with either NAD+ (5 mM), or FK866 (1 nM) or EX527 (50 μM).
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| Overall design |
mRNA was obtained from human mesenchymal stem cells (hMSC) either undifferentiatiated or at days 8 and 16 after adipogenic induction. During differentation, cells were trated with NAD+ (5 mM), FK866 (1 nM) or EX527 (50 μM). Each condition was analyzed per triplicate.
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| Contributor(s) |
Aguilar-Arnal L, Sánchez-Ramírez E |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 21, 2021 |
| Last update date |
Sep 16, 2022 |
| Contact name |
LORENA AGUILAR ARNAL |
| E-mail(s) |
loreaguilararnal@iibiomedicas.unam.mx
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| Organization name |
INSTITUTO DE INVESTIGACIONES BIOMEDICAS- UNIVERSIDAD NACIONAL AUTONOMA DE MEXICO
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| Department |
BIOLOGIA CELULAR Y FISIOLOGIA
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| Lab |
B040
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| Street address |
TERCER CIRCUITO EXTERIOR, CU
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| City |
MEXICO CITY |
| ZIP/Postal code |
04510 |
| Country |
Mexico |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (27)
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| GSM5394431 |
Adipo, Day8, R1 |
| GSM5394432 |
Adipo, Day8, R2 |
| GSM5394433 |
Adipo, Day8, R3 |
| GSM5394434 |
Adipo+NAD+,Day8, R1 |
| GSM5394435 |
Adipo+NAD+,Day8, R2 |
| GSM5394436 |
Adipo+NAD+,Day8, R3 |
| GSM5394437 |
Adipo+FK866,Day8, R1 |
| GSM5394438 |
Adipo+FK866,Day8, R2 |
| GSM5394439 |
Adipo+FK866,Day8, R3 |
| GSM5394440 |
Adipo+EX527,Day8, R1 |
| GSM5394441 |
Adipo+EX527,Day8, R2 |
| GSM5394442 |
Adipo+EX527,Day8, R3 |
| GSM5394443 |
Adipo, Day16, R1 |
| GSM5394444 |
Adipo, Day16, R2 |
| GSM5394445 |
Adipo, Day16, R3 |
| GSM5394446 |
Adipo+NAD+,Day16, R1 |
| GSM5394447 |
Adipo+NAD+,Day16, R2 |
| GSM5394448 |
Adipo+NAD+,Day16, R3 |
| GSM5394449 |
Adipo+FK866,Day16, R1 |
| GSM5394450 |
Adipo+FK866,Day16, R2 |
| GSM5394451 |
Adipo+FK866,Day16, R3 |
| GSM5394452 |
Adipo+EX527,Day16, R1 |
| GSM5394453 |
Adipo+EX527,Day16, R2 |
| GSM5394454 |
Adipo+EX527,Day16, R3 |
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| Relations |
| BioProject |
PRJNA739727 |
| SRA |
SRP324911 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE178615_fpkm_genename.xls.gz |
5.1 Mb |
(ftp)(http) |
XLS |
| GSE178615_readcount_genename.xls.gz |
2.7 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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