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Series GSE178473 Query DataSets for GSE178473
Status Public on Aug 30, 2021
Title Genetic engineering of the first functional human cardiomyocyte lines for building clinically relevant atrial fibrillation models
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Using conditional immortalization, we generated the first lines of human cardiomyocytes with preserved cardiomyogenic differentiation capacity. These human immortalized Atrial Myocytes (hiAMs) display strict control over proliferation and differentiation, allowing massive (quadrillion-fold) expansion followed by differentiation towards fully functional (i.e. excitable and contractile) human atrial cardiomyocytes. Here, we compared the transcriptome of three hiAM monoclones after differentiation (D12) with hESC-derived AMs (D28) using RNA-sequencing.
Overall design Total RNA of hiAM monoclones 2.38, 2.52 and 2.90 was sequenced after differentiation (D12). Three hiAM monoclones and four hESC-derived AM replicates were used.
Contributor(s) Harlaar N, Snabel RR, Cofiño Fabrés C, Veenstra GJ, Passier R, Pijnappels DA, de Vries AA
Citation(s) 35455744
Submission date Jun 18, 2021
Last update date Apr 27, 2022
Contact name Rebecca Regina Snabel
Organization name RIMLS
Department Molecular Developmental Biology
Lab Veenstra
Street address Geert Grooteplein 26-28
City Nijmegen
State/province Gelderland
ZIP/Postal code 6525 GA
Country Netherlands
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (7)
GSM5392289 hESC-AM1
GSM5392290 hESC-AM2
GSM5392291 hESC-AM3
BioProject PRJNA739176

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE178473_GRCh38.p13-CPM_log.tsv.gz 874.5 Kb (ftp)(http) TSV
GSE178473_GRCh38.p13-counts.tsv.gz 272.2 Kb (ftp)(http) TSV
GSE178473_metadata.tsv.gz 147 b (ftp)(http) TSV
Raw data are available in SRA
Processed data are available on Series record

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