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| Status |
Public on Aug 30, 2021 |
| Title |
Genetic engineering of the first functional human cardiomyocyte lines for building clinically relevant atrial fibrillation models |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using conditional immortalization, we generated the first lines of human cardiomyocytes with preserved cardiomyogenic differentiation capacity. These human immortalized Atrial Myocytes (hiAMs) display strict control over proliferation and differentiation, allowing massive (quadrillion-fold) expansion followed by differentiation towards fully functional (i.e. excitable and contractile) human atrial cardiomyocytes. Here, we compared the transcriptome of three hiAM monoclones after differentiation (D12) with hESC-derived AMs (D28) using RNA-sequencing.
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| Overall design |
Total RNA of hiAM monoclones 2.38, 2.52 and 2.90 was sequenced after differentiation (D12). Three hiAM monoclones and four hESC-derived AM replicates were used.
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| Contributor(s) |
Harlaar N, Snabel RR, Cofiño Fabrés C, Veenstra GJ, Passier R, Pijnappels DA, de Vries AA |
| Citation(s) |
35455744 |
| |
| Submission date |
Jun 18, 2021 |
| Last update date |
Apr 27, 2022 |
| Contact name |
Rebecca Regina Snabel |
| Organization name |
RIMLS
|
| Department |
Molecular Developmental Biology
|
| Lab |
Veenstra
|
| Street address |
Geert Grooteplein 26-28
|
| City |
Nijmegen |
| State/province |
Gelderland |
| ZIP/Postal code |
6525 GA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA739176 |
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