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| Status |
Public on Sep 10, 2021 |
| Title |
A Single-Cell Atlas of Liver Metastases of Colorectal Cancer Reveals the Reprogramming of the Tumor Microenvironment in Response to Preoperative Chemotherapy |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Metastasis is the primary cause of cancer-related mortality in colorectal cancer (CRC) patients. How to improve therapeutic options for patients with metastatic CRC (mCRC) is the core question for CRC treatment. However, the complexity and diversity of stromal context of the tumor microenvironment (TME) in liver metastases of CRC is not fully understood, and its influence on response to chemotherapy is unclear. Here we provide an in-depth analysis of transcriptional landscape of primary CRC, matched liver metastases and blood at single-cell resolution, and perform a systematic examination of transcriptional changes and phenotypic alteration of the TME in response to preoperative chemotherapy (PC). Based on 111,292 single-cell transcriptomes, our study reveals that TME of treatment-naïve tumors is characterized by higher abundance of less-activated B cells and higher heterogeneity of tumor-associated macrophages (TAMs). By contrast, in tumors treated with PC, we find activation of B cells, lower diversity of TAMs with immature and less activated phenotype, lower abundance of both dysfunctional T cells and ECM-remodeling cancer-associated fibroblasts (CAFs), and an accumulation of myofibroblasts. Our study provides a foundation for future investigation of the cellular mechanisms underlying liver metastasis of CRC and its response to PC, and opens up new possibilities for the development of therapeutic strategies for CRC.
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| Overall design |
Single-cell expression profiling of the liver metastases of CRC in treatment patients and untreated patients.
For data usage terms and conditions, please contact the principle investigator (Jianming Li, pathologylee@126.com).
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| Contributor(s) |
Li J, Che L |
| Citation(s) |
34489408 |
| BioProject |
PRJNA725335 |
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| Submission date |
Jun 16, 2021 |
| Last update date |
Sep 20, 2021 |
| Contact name |
liheng che |
| E-mail(s) |
cheliheng@126.com
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| Organization name |
Sun Yat sen university
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| Street address |
Xin gang road
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| City |
Guangzhou |
| ZIP/Postal code |
510000 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (15)
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| GSM5387660 |
scRNA-seq of liver metastases of colorectal cancer:COL07 primary CRC |
| GSM5387661 |
scRNA-seq of liver metastases of colorectal cancer:COL07 liver metastases |
| GSM5387662 |
scRNA-seq of liver metastases of colorectal cancer:COL12 primary CRC |
| GSM5387663 |
scRNA-seq of liver metastases of colorectal cancer:COL12 liver metastases |
| GSM5387664 |
scRNA-seq of liver metastases of colorectal cancer:COL12 PBMC |
| GSM5387665 |
scRNA-seq of liver metastases of colorectal cancer:COL15 primary CRC |
| GSM5387666 |
scRNA-seq of liver metastases of colorectal cancer:COL15 liver metastases |
| GSM5387667 |
scRNA-seq of liver metastases of colorectal cancer:COL16 primary CRC |
| GSM5387668 |
scRNA-seq of liver metastases of colorectal cancer:COL16 liver metastases |
| GSM5387669 |
scRNA-seq of liver metastases of colorectal cancer:COL17 primary CRC |
| GSM5387670 |
scRNA-seq of liver metastases of colorectal cancer:COL17 liver metastases |
| GSM5387671 |
scRNA-seq of liver metastases of colorectal cancer:COL17 PBMC |
| GSM5387672 |
scRNA-seq of liver metastases of colorectal cancer:COL18 primary CRC |
| GSM5387673 |
scRNA-seq of liver metastases of colorectal cancer:COL18 liver metastases |
| GSM5387674 |
scRNA-seq of liver metastases of colorectal cancer:COL18 PBMC |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE178318_barcodes.tsv.gz |
578.6 Kb |
(ftp)(http) |
TSV |
| GSE178318_genes.tsv.gz |
258.3 Kb |
(ftp)(http) |
TSV |
| GSE178318_matrix.mtx.gz |
520.7 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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