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Status |
Public on Mar 29, 2022 |
Title |
Transcriptomes of crypt intestinal epithelial cells from Apln-/- mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We used bulk RNAseq to analyze crypt intestinal epithelial gene expression changes between wild-type and Apln-/- adult mice
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Overall design |
Small intestines from 13 week old, male, wild-type control (C57Bl/6) or Apln-/- mice were harvested and digested with Liberase TL and crypt epithelial cells were sorted as single, live, CD31neg, CD45neg, EpCAM+, CD44+ cells. RNA was reverse transcribed, amplified and sequenced.
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Contributor(s) |
Bernier-Latmani J, Dupanloup I, Delorenzi M, Petrova TV |
Citation(s) |
35602406 |
Submission date |
Jun 14, 2021 |
Last update date |
Aug 23, 2022 |
Contact name |
Isabelle Dupanloup |
E-mail(s) |
isabelle.dupanloup@unige.ch
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Organization name |
University of Geneva
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Street address |
Rue Michel-Servet, 1
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City |
Geneva |
ZIP/Postal code |
1206 |
Country |
Switzerland |
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Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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Samples (6)
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GSM5380294 |
Sorted intestinal crypt epithelial cells from wild-type adult mice, biological replicate 1 |
GSM5380295 |
Sorted intestinal crypt epithelial cells from wild-type adult mice, biological replicate 2 |
GSM5380296 |
Sorted intestinal crypt epithelial cells from wild-type adult mice, biological replicate 3 |
GSM5380297 |
Sorted intestinal crypt epithelial cells from Apln-/- adult mice, biological replicate 1 |
GSM5380298 |
Sorted intestinal crypt epithelial cells from Apln-/- adult mice, biological replicate 2 |
GSM5380299 |
Sorted intestinal crypt epithelial cells from Apln-/- adult mice, biological replicate 3 |
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This SubSeries is part of SuperSeries: |
GSE178118 |
Transcriptomes of crypt intestinal epithelial cells from Apln-/- mice |
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Relations |
BioProject |
PRJNA737406 |
SRA |
SRP323946 |