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Series GSE17786 Query DataSets for GSE17786
Status Public on Aug 25, 2009
Title Response of ethanologenic E coli strains LY180 and EMFR9 to furfural
Platform organism Escherichia coli str. K-12 substr. MG1655
Sample organism Escherichia coli
Experiment type Expression profiling by array
Summary The data explore the transcriptional response of strain LY180 and the furfural-resistant derivative EMFR9 to 0.5 g/L furfural
LY180 and EMFR9 and differences in their expression profiles are described in Miller, E. N., L. R. Jarboe, L. P. Yomano, S. W. York, K. T. Shanmugam, and L. O. Ingram. 2009. Silencing of NADPH-dependent oxidoreductase genes (yqhD and dkgA) in furfural-resistant ethanologenic Escherichia coli. Appl Environ Microbiol 75:4315-23. The response of LY180 to furfural is described in Miller, E. N., L. R. Jarboe, P. C. Turner, P. Pharkya, L. P. Yomano, S. W. York, D. Nunn, K. T. Shanmugam, and L. O. Ingram. 2009. Furfural Inhibits Growth by Limiting Sulfur Assimilation in Ethanologenic Escherichia coli strain LY180. Appl Environ Microbiol., 2009.
Overall design Total RNA was prepared from cultures of LY180 and EMFR9 immediately before and 15 min after addition of furfural to 0.5 g/L. The Nimblegen TI83333 chip measures expression of 4,237 genes, with 5 replicates, and 18 probes average per gene.

Data from replicate 1 (OID 8641) and replicate 2 (OID 14117) was analyzed independently.
Contributor(s) Miller EN, Jarboe LJ, Turner PC, Ingram LO
Citation(s) 19684179, 19429550
Submission date Aug 24, 2009
Last update date Apr 27, 2013
Contact name Peter Christopher Turner
Phone 352-846-0955
Organization name University of Florida
Department Micro and Cell Science
Lab Ingram
Street address Bldg 981, Museum Road
City Gainesville
State/province FL
ZIP/Postal code 32611-0700
Country USA
Platforms (1)
GPL9088 NimbleGen Escherichia coli K12 TI83333 (385K)
Samples (8)
GSM443990 E coli LY180_rep1
GSM443991 E coli LY180_w 0.5g/L Furfural_rep1
GSM443992 E coli EMFR9_rep1
BioProject PRJNA117885

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Supplementary file Size Download File type/resource
GSE17786_RAW.tar 72.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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