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Series GSE17669 Query DataSets for GSE17669
Status Public on Sep 30, 2009
Title Gene expression in the barley spike during drought stress
Organism Hordeum vulgare
Experiment type Expression profiling by array
Summary The photosynthetic organs of the barley spike (lemma, palea and awn) are resistant to drought. This is a beneficial trait because they can sustain grain-filling when drought occurs at the reproductive stage. There is little information about gene expression in the spike organs under drought conditions. In this study, we compared gene expression in drought-stressed lemma, palea, awn and seed at the grain-filling stage using the Barley1 Genome Array in order to identify drought-regulated organ-specific genes.
 
Overall design Barley plants were drough-stressed for 4 days at the grain-filling stage by withholding water. Lemma, palea, awn and seed were collected to determine gene expression during drought in each organ. For each organ three biological replications were collected on three different dates. On each of the three dates, one replication of each organ was collected from plants grown in separate pots using a randomized complete block design (RCBD). In ths design, organ type and drought treatment were treatment effects and date of sample collection (replication) was the block. To get enough tissue for RNA extraction, each replication for each organ was collected from four plants in the same pot. RNA from each replicate sample was hybridized to individual Barley1 GeneChips. Thus, for four organs with three replications (blocks) and two treatment levels, a total of 24 Barley1 GeneChips were used.
 
Contributor(s) Abebe T
Citation(s) 19936810
Submission date Aug 17, 2009
Last update date Mar 21, 2012
Contact name Tilahun Abebe
E-mail(s) Tilahun.Abebe@uni.edu
Phone 319-273-7151
Organization name University of Northern Iowa
Department Biology
Street address 191 McCollum Hall
City Cedar Falls
State/province IA
ZIP/Postal code 50614
Country USA
 
Platforms (1)
GPL1340 [Barley1] Affymetrix Barley Genome Array
Samples (24)
GSM440957 Control lemma_Rep1
GSM440958 Control lemma_Rep2
GSM440959 Control lemma_Rep3
Relations
BioProject PRJNA118475

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17669_RAW.tar 53.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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