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| Status |
Public on Jun 09, 2021 |
| Title |
Genome-wide effect of STING-mediated activation in T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Using wild type mice and mice deficient forSTING , we compared genome-wide expressionof RNA in response to STING activation with DMXAA
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| Overall design |
To look for the role of STING-mediated pathway in activation of T cells, we activated naïve T cells with DMXAA, a synthetic agonist of STING, together with activation of TCR. To define a STING-specific effect, STING-deficient animals were used as a control. We isolated naïve T cells from peripheral lymph nodes, negatively selected them using magnetic beads coated with antibodies, and activated cells for 4 hours in specified conditions. Next, we isolated RNA and made library using TruSeq kit (Illumina) for the library construction.One replica of each sample is provided
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| Contributor(s) |
Poltorak A, Larkin B |
| Citation missing |
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| |
| Submission date |
Jun 08, 2021 |
| Last update date |
Jun 09, 2021 |
| Contact name |
Alexander Poltorak |
| E-mail(s) |
triftorak@gmail.com
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| Phone |
6176363596
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| Organization name |
tufts
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| Street address |
150 Harrison avenue, J529
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA736030 |
| SRA |
SRP323223 |
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