|
| Status |
Public on Jan 07, 2022 |
| Title |
H3K36me3 profiling in mTEClo |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
AIRE-sensitive genes are marked with low levels of H3K36me3 in mTEClo
|
| |
|
| Overall design |
Nano-ChIP-seq on H3K36me3 was performed as previously described (Adli and Bernstein, 2011) on 5*10^4 purified mTEClo (EpCAM+ UEA-1+ Ly51lo CD80lo) from individual B6 mice. ChIP-seq libraries were prepared with the TruSeq ChIP Sample Preparation Kit (Illumina) and 2x75-bp paired-end reads were sequenced on an Illumina HiSeq 2000 machine.
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| |
|
| Contributor(s) |
Irla M, Giraud M |
| Citation(s) |
35037357 |
| |
| Submission date |
Jun 08, 2021 |
| Last update date |
Jan 24, 2022 |
| Contact name |
Matthieu Giraud |
| E-mail(s) |
matthieu.giraud@inserm.fr
|
| Organization name |
INSERM _ Université de Nantes
|
| Department |
CRTI
|
| Street address |
30 bd Jean Monnet
|
| City |
Nantes |
| ZIP/Postal code |
44000 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA736029 |
| SRA |
SRP323209 |
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