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Series GSE176129 Query DataSets for GSE176129
Status Public on Nov 10, 2021
Title Integrated molecular analysis identifies new developmental pericyte markers in zebrafish
Organism Danio rerio
Experiment type Expression profiling by high throughput sequencing
Summary We performed single cell and bulk RNA-seq analyses on pdgfrb-positive cells to identify candidate pericyte-expressed genes. For bulk RNA-seq, we used wild type pdgfrb-positive samples from Series GSE152759 (GEO ID: GSM4625924, GSM4625925, GSM4625926) for comparison to mutant pdgfrb-positive cells in this Series. These cells had originally been isolated in parallel.
Overall design RNA-seq was performed on transgene-positive cells isolated by FACS.
Contributor(s) Lawson N
Citation(s) 34751773
NIH grant(s)
Grant ID Grant title Affiliation Name
R35 HL140017 Embryonic origins of endothelial heterogeneity UNIV OF MASSACHUSETTS MED SCHOOL NATHAN D LAWSON
Submission date Jun 03, 2021
Last update date Nov 12, 2021
Contact name Nathan D. Lawson
Organization name University of Massachusetts Medical School
Department Molecular, Cell, and Cancer Biology
Street address 364 Plantation Street
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
Platforms (2)
GPL14875 Illumina HiSeq 2000 (Danio rerio)
GPL21741 Illumina HiSeq 4000 (Danio rerio)
Samples (5)
GSM5356646 pdgfrb-positive/um148 mutant rep1
GSM5356647 pdgfrb-positive/um148 mutant rep2
GSM5356648 pdgfrb-positive/um148 mutant rep3
BioProject PRJNA734950
SRA SRP322535

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE176129_RAW.tar 110.9 Mb (http)(custom) TAR (of MTX, TSV)
GSE176129_alldetectedWtvUm148.csv.gz 1.7 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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