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| Status |
Public on Jun 03, 2021 |
| Title |
Reelin and Thrombospondin1 regulate cardiomyocyte proliferation through Vldlr |
| Organisms |
Mus musculus; Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed RNA-Seq analysis of neoatal rat ventricular cardiomyocytes (NRVCs)which were treated with Reelin,or knocking down Vldlr,Thbs1,or overexpressing Vldlr to investigate the moleclular mechanism of Thbs1/Reelin-Vldlr to regulate cardiomyocyte proliferation.In addition,the day 1mouse cardiomyocytes in Myh6-Cre;Vldlr f/f mice ,Myh6-Cre;Vldlr f/+ mice ,versus Vldlr f/f mice were also performed to explore the fucntion of Vldlr which regulating cardiomyocyte proliferation in vivo.
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| Overall design |
RNA-Seq analysis of NRVCs which adminstration of Reelin, koncking down Vldlr ,Thbs1,overexpressing Vldlr,2 replicates per group and RNA-Seq analysis of day 1 mouse cardiomyocytes in Myh6-Cre;Vldlr f/f mice, Myh6-Cre;Vldlr f/+ mice and Vldlr f/f mice,3 replicates per group.
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| Contributor(s) |
Pei L, Zhang H, Wei K |
| Citation missing |
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| |
| Submission date |
Jun 02, 2021 |
| Last update date |
Jun 03, 2021 |
| Contact name |
Lijuan Pei |
| E-mail(s) |
lijuanpss3@gmail.com
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| Phone |
19802102306
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| Organization name |
Tongji University
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| Street address |
CHIFENG ROAD 67
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| City |
Shanghai |
| ZIP/Postal code |
200082 |
| Country |
China |
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| Platforms (2) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (25)
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| Relations |
| BioProject |
PRJNA734577 |
| SRA |
SRP322342 |
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