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| Status |
Public on Jun 01, 2021 |
| Title |
RNA sequencing comparing in-vitro lentiviral CRISPR-Cas9 knockdown of Non-Canonical Polycomb Repressive Complex 1.6 members from a Kras G12D Trp53 -/- background mouse lung cancer cell line |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The goal of this study was to compare expression profiles of mouse Kras G12D Trp53 -/- lung cancer cells that have inactivated ncPRC1.6 components to a ncPRC1.6 wild type condition
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| Overall design |
Each condition (Empty vs sg_ncPRC1.6 member (either sgMga, sgL3mbtl2, sgPcgf6, or sgL3mbtl2+sgPcgf6 cotransduced) is represented in 1 control Empty line in biological triplicate, 1 sgMga line in biological triplicates, 1 sgL3mblt2 line in biological triplicate, 1sgPcgf6 in biological triplicate, and 1 sgL3mbtl2+sgPcg6 co-transduced line in biological triplicate.
ChiP-Seq comparing binding of various Non-Canonical Polycomb Repressive Complex 1.6 members and Pol II between WT MGA and MGA KO in cells derived from Kras G12D + Trp53 -/- mouse tumours.
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| Contributor(s) |
Mathsyaraja H, Eisenman RN |
| Citation(s) |
34236315 |
| |
| Submission date |
May 31, 2021 |
| Last update date |
Jul 20, 2021 |
| Contact name |
Haritha Mathsyaraja |
| E-mail(s) |
hmathsya@fredhutch.org
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| Organization name |
Fred Hutch
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| Department |
Basic Sciences
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| Lab |
Robert N Eisenman
|
| Street address |
1100 Fairview Ave N
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA734014 |
| SRA |
SRP322007 |
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