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Series GSE17513 Query DataSets for GSE17513
Status Public on Sep 01, 2009
Title Expression data from murine embryonic stem cell derived cardiac progenitors
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mamamlian cardiogenesis occurs through the development of discreate populations of first and second heart field progenitors. We have used a dual transgenic color reproter system to isolate purified populations of these progenitors.

We used microarrays to detail the global programme of gene expression underlying cardiac development in mouse

All four populations of cells are derived from embryonic stem cells differentiating in vitro (day 6 of in vitro differentaition). The stem cell line has two transgenic reporters as follows:

1. The second heart field (SHF) specific reporter of the Mef2C gene (E. Dodou, S. M. Xu, B. L. Black, Mech Dev 120, 1021 (Sep, 2003)) driving the expression of dsRed

2. The cardiac specific enhancer ( C. L. Lien et al., Development 126, 75 (Jan, 1999)) driving the expression of eGFP. Thus, the red cells are SHF specific, the green cells are cardiac specific, and the red/green are SHF and cardiac specific. These cells are compared to the double negative cells which serve as a control.
 
Overall design Embryonic stem cell derived progenitors were isolated into four distinct populations by FACS purifying these progenitors based on a two color reporter system. Four populations were then compared to each other by transcriptional profiling.
 
Contributor(s) Domian I, Chiravuri M
Citation(s) 19833966
Submission date Aug 05, 2009
Last update date Feb 11, 2019
Contact name Ibrahim J Domian
E-mail(s) idomian@partners.org
Organization name Massachusetts General Hospital
Department Division of Cardiology
Lab Domian
Street address 185 Cambridge Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM436530 Red+/Green+ progenitor, experiment A
GSM436531 Red+/Green- progenitor, experiment A
GSM436532 Red-/Green+ progenitor, experiment A
Relations
BioProject PRJNA118785

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17513_RAW.tar 34.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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