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| Status |
Public on Jan 21, 2022 |
| Title |
Distinct MUNC lncRNA structural domains regulate transcription of different promyogenic factors [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Many lncRNAs have been discovered using transcriptomic data, however, it is unclear what fraction of lncRNAs is functional and what structural properties affect their phenotype. MUNC lncRNA (also known as DRReRNA) acts as an enhancer RNA for the Myod1 gene in cis and stimulates the expression of other promyogenic genes in trans by recruiting the cohesin complex. Here, experimental probing of the RNA structure revealed that MUNC contains multiple structural domains not detected by prediction algorithms in the absence of experimental information. We show that these specific and structurally distinct domains are required for induction of promyogenic genes, for binding genomic sites and gene expression regulation, and for binding the cohesin complex. Myod1 induction and cohesin interaction comprise only a subset of MUNC phenotype. Our study reveals unexpectedly complex, structure-driven functions for the MUNC lncRNA and emphasizes the importance of experimentally determined structures for understanding structure-function relationships in lncRNAs.
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| Overall design |
RNA-seq on 18 samples; control C2C12 cells, MUNC spliced overexpressing cells, and MUNC genomic overexpressing cells; proliferation and differentiation conditions; 3 biological replicates each
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| Contributor(s) |
Przanowska RK, Przanowski P, Guertin M, Dutta A |
| Citation(s) |
35172143 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AR067712 |
Mechanism of action of an lncRNA for directing muscle differentiation |
UNIVERSITY OF VIRGINIA CHARLOTTESVILLE |
Anindya Dutta |
| R35 GM128635 |
Mechanisms of coordinate gene regulation by transcription factors |
UNIVERSITY OF CONNECTICUT HEALTH CENTER |
Michael Joseph Guertin |
| F99 CA253732 |
Long non-coding RNAs as novel therapeutic targets in gliomas treatment |
UNIVERSITY OF VIRGINIA CHARLOTTESVILLE |
Roza Kamila Przanowska |
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| |
| Submission date |
May 10, 2021 |
| Last update date |
Mar 31, 2022 |
| Contact name |
Roza Kamila Przanowska |
| Organization name |
University of Virginia
|
| Department |
Biomedical Engineering
|
| Street address |
1340 JPA
|
| City |
Charlottesville |
| State/province |
Virginia |
| ZIP/Postal code |
22908 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE174218 |
Distinct MUNC lncRNA structural domains regulate transcription of different promyogenic factors |
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| Relations |
| BioProject |
PRJNA728732 |
| SRA |
SRP319240 |
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