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| Status |
Public on May 30, 2021 |
| Title |
Vector-borne Trypanosoma brucei parasites develop and persist in artificial human skin as skin tissue forms |
| Organisms |
Trypanosoma brucei; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transmission of Trypanosoma brucei by tsetse flies involves the deposition of the infective quiescent metacyclic stage into the mammalian skin at the site of the fly’s bite. In the skin, the metacyclic parasites reactivate and differentiate into proliferative trypanosomes before colonizing the host's blood and tissues. We have generated an advanced human skin equivalent and used tsetse flies to naturally infect the artificial skin with trypanosomes. We have detailed the chronological order of the parasites' development in the skin and found a rapid activation and differentiation of the tsetse-transmitted cell cycle‑arrested metacyclic trypanosomes to proliferative parasites. Single-parasite transcriptomics documented the biological events during differentiation and host invasion at five different time points. After the establishment of a proliferative trypanosome population in the skin, the parasites entered a reversible quiescence program characterized by slow replication and a strongly reduced metabolism. We termed these quiescent trypanosomes skin tissue forms (STF), which may play an important role in maintaining the trypanosome infection in aparasitemic, asymptomatic individuals.
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| Overall design |
After natural infection, skin equivalents were cultured for another 4 h, 12 h, 24 h, and 7 d, respectively. In addition, freshly collected MCFs obtained from tsetse flies were used. Skin equivalents were dissociated and single viable parasites were sorted based on their tdTomato and Calcein AM signal with a FACSAria III cytometer (BD Biosciences) into 48-well plates containing 2.6 µl of 1x lysis buffer (Takara) supplemented with 0.01 µl of RNase inhibitor (40 U/µl; Takara). Immediately after sorting, cells were placed on ice for 5 min and stored at -80 °C. Library preparation and sequencing was carried out as described previously (Muller et al., 2018). Briefly, 0.2 µl of a 1:20 x 106 dilution of ERCC Spike-in Control Mix 1 (Thermo Fisher Scientific) were added to the lysates of single parasites and libraries were prepared using SMART-Seq v.4 Ultra Low Input RNA Kit (Takara) using a quarter of the reagent volumes recommended by the manufacturer. 27 cycles were used for PCR amplification and cDNA was purified using Agencourt AMPure XP beads (Beckman Coulter) using 15 μl of elution buffer (Takara). Library quantification was performed with a Qubit 3 Fluorometer with dsDNA Hs Assay kit (Life Technologies) and the quality of the libraries was assessed using a 2100 Bioanalyzer with High Sensitivity DNA kit (Agilent). 0.5 ng of cDNA was subjected to a tagmentation-based protocol (Nextera XT, Illumina) using a quarter of the recommended volumes, 10 min for tagmentation at 55 °C and 1 min extension time during PCR amplification. Libraries were pooled and sequencing was performed in paired-end mode for 2 × 75 cycles using Illumina’s NextSeq 500.
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| Contributor(s) |
Reuter C, Imdahl F, Hauf L, Vafadarnejad E, Fey P, Finger T, Walles H, Saliba A, Groeber-Becker F, Engstler M |
| Citation(s) |
37996412 |
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https://doi.org/10.1101/2021.05.13.443986
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| Submission date |
May 10, 2021 |
| Last update date |
Dec 08, 2023 |
| Contact name |
Antoine-Emmanuel Saliba |
| E-mail(s) |
emmanuel.saliba@helmholtz-hzi.de
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| Phone |
+49-931-31-81341
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| Organization name |
Helmholtz Institute for RNA-based Infection Research
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| Street address |
Josef-Schneider-Straße 2 / D15
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| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
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| Platforms (2) |
| GPL20572 |
Illumina NextSeq 500 (Trypanosoma brucei) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (186)
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| Relations |
| BioProject |
PRJNA728721 |
| SRA |
SRP319235 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE174198_RAW.tar |
1.4 Gb |
(http)(custom) |
TAR (of H5, MTX, TSV) |
| GSE174198_count_table_bsf_processed.csv.gz |
199.4 Kb |
(ftp)(http) |
CSV |
| GSE174198_count_table_bsf_unprocessed.csv.gz |
205.5 Kb |
(ftp)(http) |
CSV |
| GSE174198_single_trypanosomes_normalized_reads.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
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