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| Status |
Public on Apr 01, 2024 |
| Title |
Transcriptome analysis of endometrium cells in Equus asinus |
| Organism |
Equus asinus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Deoxynivalenol (DON) and zearalenone (ZEA) are the most common mycotoxins, which are damage to the reproductive system of human and animal. Endometrium cells perform vital roles of female reproduction. In the present study, transcriptomic was conducted on different dosage (0 μM, 10 μM and 30 μM) of DON and ZEA treatment donkey endometrium cells. Transcriptomic analysis showed 7386 differentially expressed genes (DEGs) were identified between DON 10 μM treatment group and control group, with 3655 up regulated and 3731 down; 8694 DEGs were identified between DON 30 μM treatment group and control group, with 4532 up regulated and 4162 down; 1768 DEGs were identified between ZEA 10 μM treatment group and control group, with 763 up regulated and 1005 down; and 3311 DEGs were identified between ZEA 30 μM treatment group and control group, with 1501 up regulated and 1810 down. In DEGs, Caspase-1, GSDMD-NT, ESR 1, PRDX 4, NOX 1, MSH 6 and CD 44, were expressed changed in DON and ZEA treatment group, which related to pyroptosis, hormone secretion, oxidative stress and cancerous. It is unprecedented that DON induced cell death by pyroptosis. This study provides valuable genetic resources for understanding the mechanisms and pathways of the toxin effect of DON and ZEA for Equus asinus’ endometrium cells.
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| Overall design |
Total mRNA profiles of DON and ZEA treated endometrium cells were generated by deep sequencing, in triplicate.
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| Contributor(s) |
Zhang G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 06, 2021 |
| Last update date |
Apr 01, 2024 |
| Contact name |
JunLin Song |
| E-mail(s) |
songjunlin123@sina.com
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| Organization name |
JunLin Song
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| Street address |
Changcheng Road
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| City |
Qingdao |
| ZIP/Postal code |
266109 |
| Country |
China |
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| Platforms (1) |
| GPL30098 |
Illumina HiSeq 2000 (Equus asinus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA728195 |
| SRA |
SRP319022 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE174022_Equus_asinus_NCBI_novel_gene.fa.gz |
1.1 Mb |
(ftp)(http) |
FA |
| GSE174022_gene.fa.gz |
22.3 Mb |
(ftp)(http) |
FA |
| GSE174022_gene_fpkm.xls.gz |
4.9 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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