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| Status |
Public on Jun 30, 2021 |
| Title |
Notch1 switches progenitor competence in inducing medulloblastoma |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The identity of the cell of origin is a key determinant of cancer subtype, progression and prognosis. Group 3 medulloblastoma (MB) is a malignant childhood brain cancer with poor prognosis and few candidates as putative cell of origin. We overexpressed the Group 3 MB genetic drivers MYC and Gfi1 in different candidate cells of origin in the postnatal mouse cerebellum. We found that S100b+ cells are competent to initiate Group 3 MB and we observed that S100b+ cells have higher levels of Notch1 pathway activity compared to Math1+ cells. Interestingly, we found that additional activation of Notch1 in Math1+ and Sox2+ cells was sufficient to induce Group 3 MB upon MYC/Gfi1 expression. Taken together, our data suggest that the Notch1 pathway plays a critical role in Group 3 MB initiation.
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| Overall design |
RNA-sequencing profiles of medulloblastoma samples induced by N1ICD+MYC/Gfi1 (in Math1+ cells) and MYC/Gfi1 (in S100b+ cells) compared with previously published Group 3 MB mouse models (MYC/Gfi1, MYC/Otx2)
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| |
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| Contributor(s) |
Ballabio C, Gianesello M, Okonechnikov K, Tiberi L |
| Citation(s) |
34162555 |
| |
| Submission date |
May 05, 2021 |
| Last update date |
Jul 02, 2021 |
| Contact name |
Luca Tiberi |
| E-mail(s) |
Luca.tiberi@unitn.it
|
| Phone |
+390461285356
|
| Organization name |
University of Trento
|
| Department |
Department of Cellular, Computational and Integrative Biology - CIBIO
|
| Lab |
Armenise-Harvard Laboratory of Brain Disorders and Cancer
|
| Street address |
via Sommarive, 9
|
| City |
Trento |
| State/province |
TN |
| ZIP/Postal code |
38123 |
| Country |
Italy |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (11)
|
| GSM5282388 |
Medulloblastoma MYC-Otx2_rep2 |
| GSM5282389 |
Medulloblastoma MYC-Otx2_rep3 |
| GSM5282390 |
Medulloblastoma MYC-Gfi1 (in S100b+ cells)_rep1 |
| GSM5282391 |
Medulloblastoma MYC-Gfi1 (in S100b+ cells)_rep2 |
| GSM5282392 |
Medulloblastoma MYC-Gfi1 (in S100b+ cells)_rep3 |
| GSM5282393 |
Medulloblastoma MYC-Gfi1-N1ICD (in Math1+ cells)_rep1 |
| GSM5282394 |
Medulloblastoma MYC-Gfi1-N1ICD (in Math1+ cells)_rep2 |
| GSM5282395 |
Medulloblastoma MYC-Gfi1-N1ICD (in Math1+ cells)_rep3 |
|
| Relations |
| BioProject |
PRJNA727475 |
| SRA |
SRP318533 |
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