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Series GSE173472 Query DataSets for GSE173472
Status Public on Dec 15, 2021
Title Transcriptomic analysis of gnotobiotic Aedes aegypti mosquitoes
Organism Aedes aegypti
Experiment type Expression profiling by high throughput sequencing
Summary We compare the transcriptome of gnotobiotic Ae. aegypti generated by contaminating axenic (bacteria-free) larvae with bacterial isolates found in natural mosquito breeding sites. We focused on four bacterial isolates (Lysobacter, Flavobacterium, Paenibacillus and Enterobacteriaceae) and found that different gnotobiotic treatments resulted in massive transcriptomic changes throughout the mosquito development.
 
Overall design 48 samples, 3 mosquito stages (larval, pupal and adult stages), 5 conditions, 3 replicates of each type of sample. The non-axenic samples were the reference sample at each stage.
 
Contributor(s) Giraud E, Sismeiro O, Varet H, Legendre R, Lambrechts L
Citation(s) 34905257
Submission date Apr 28, 2021
Last update date Dec 15, 2021
Contact name Rachel Legendre
E-mail(s) rachel.legendre@pasteur.fr
Organization name Institut Pasteur
Department Research and Resource Center for Scientific Informatics
Lab Hub of Bioinformatics and Biostatistics
Street address 28, rue du docteur Roux
City Paris
ZIP/Postal code 75724
Country France
 
Platforms (1)
GPL19664 Illumina HiSeq 2500 (Aedes aegypti)
Samples (48)
GSM5268326 Axenic larvae rep1 [ax.lar1]
GSM5268327 Axenic larvae rep2 [ax.lar2]
GSM5268328 Axenic larvae rep3 [ax.lar3]
Relations
BioProject PRJNA725701
SRA SRP316698

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE173472_Aaegyti.complete.txt.gz 13.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record
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