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Series GSE17316 Query DataSets for GSE17316
Status Public on Nov 23, 2009
Title Reprogramming of a B cell line into macrophages
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transcription factor induced reprogramming of one specialized cell type into another is a promising approach for regenerative medicine. However, the process still remains poorly understood, in large part because of the lack of adequate experimental models. Here we describe a robust cell reprogramming system consisting of a B cell line with an inducible form of C/EBPa that can be converted into macrophages with essentially 100% efficiency in only 2 to 3 days. The conversion involves reciprocal changes in cell surface antigen expression, increase in cell granularity and size, alterations in cellular structures, formation of membrane extensions, acquisition of phagocytic capacity and an increased inflammatory responsiveness as well as migratory activity. Analysis of the transcriptome shows complex reciprocal regulation of B cell and macrophage genes, including transcription factors required for the formation of the two lineages. The fact that the cells become irreversibly committed to a macrophage fate within 1 to 2 days after activation of C/EBPa show that they are truly reprogrammed. The system should be useful to study epigenetic and cell biological mechanisms of transcription factor induced cell reprogramming.
 
Overall design time course analysis.
6 duplicated conditions: 12 samples.
control: HAFTL cells
time points: vehicle control, 3h, 12h, 24h, 48h.
 
Contributor(s) Bussmann L, Schubert A, Vu Manh T, De Andres L, Desbordes S, Parra M, Zimmermann T, Rapino F, Graf T
Citation(s) 19896445
Submission date Jul 24, 2009
Last update date Feb 11, 2019
Contact name Thomas GRAF
E-mail thomas.graf@crg.es
Organization name Center for Genomic Regulation (CRG)
Street address Dr.Aiguader, 88
City Barcelona
ZIP/Postal code 08003
Country Spain
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM433168 HAFTL cell line treated with beta estradiol sample 1
GSM433169 HAFTL cell line treated with beta estradiol sample 2
GSM433172 C10 cell line with an inducible form of C/EBPa, treated with ethanol sample 1
Relations
BioProject PRJNA119041

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17316_RAW.tar 42.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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