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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 23, 2009 |
| Title |
Reprogramming of a B cell line into macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Transcription factor induced reprogramming of one specialized cell type into another is a promising approach for regenerative medicine. However, the process still remains poorly understood, in large part because of the lack of adequate experimental models. Here we describe a robust cell reprogramming system consisting of a B cell line with an inducible form of C/EBPa that can be converted into macrophages with essentially 100% efficiency in only 2 to 3 days. The conversion involves reciprocal changes in cell surface antigen expression, increase in cell granularity and size, alterations in cellular structures, formation of membrane extensions, acquisition of phagocytic capacity and an increased inflammatory responsiveness as well as migratory activity. Analysis of the transcriptome shows complex reciprocal regulation of B cell and macrophage genes, including transcription factors required for the formation of the two lineages. The fact that the cells become irreversibly committed to a macrophage fate within 1 to 2 days after activation of C/EBPa show that they are truly reprogrammed. The system should be useful to study epigenetic and cell biological mechanisms of transcription factor induced cell reprogramming.
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| Overall design |
time course analysis.
6 duplicated conditions: 12 samples.
control: HAFTL cells
time points: vehicle control, 3h, 12h, 24h, 48h.
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| Contributor(s) |
Bussmann L, Schubert A, Vu Manh T, De Andres L, Desbordes S, Parra M, Zimmermann T, Rapino F, Graf T |
| Citation(s) |
19896445 |
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| Submission date |
Jul 24, 2009 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Thomas GRAF |
| E-mail(s) |
thomas.graf@crg.es
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| Organization name |
Center for Genomic Regulation (CRG)
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| Street address |
Dr.Aiguader, 88
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| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM433168 |
HAFTL cell line treated with beta estradiol sample 1 |
| GSM433169 |
HAFTL cell line treated with beta estradiol sample 2 |
| GSM433172 |
C10 cell line with an inducible form of C/EBPa, treated with ethanol sample 1 |
| GSM433173 |
C10 cell line with an inducible form of C/EBPa, treated with ethanol sample 2 |
| GSM433178 |
C10 cell line with an inducible form of C/EBPa, treated with beta estradiol for 3 hours sample 1 |
| GSM433181 |
C10 cell line with an inducible form of C/EBPa, treated with beta estradiol for 3 hours sample 2 |
| GSM433183 |
C10 cell line with an inducible form of C/EBPa, treated with beta estradiol for 12 hours sample 1 |
| GSM433184 |
C10 cell line with an inducible form of C/EBPa, treated with beta estradiol for 12 hours sample 2 |
| GSM433185 |
C10 cell line with an inducible form of C/EBPa, treated with beta estradiol for 24 hours sample 1 |
| GSM433186 |
C10 cell line with an inducible form of C/EBPa, treated with beta estradiol for 24 hours sample 2 |
| GSM433189 |
C10 cell line with an inducible form of C/EBPa, treated with beta estradiol for 48 hours sample 1 |
| GSM433204 |
C10 cell line with an inducible form of C/EBPa, treated with beta estradiol for 48 hours sample 2 |
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| Relations |
| BioProject |
PRJNA119041 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE17316_RAW.tar |
42.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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