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| Status |
Public on Apr 23, 2021 |
| Title |
Mapping genotype-expression associations in Heterogeneous Stock rat brains to advance behavioral genetics research [Nucleus accumbens core] |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Research into the genetic influences of impulsivity and reward motivated behavior relies heavily on outbred animal populations, including Heterogeneous Stock (HS) rats, for the genetic diversity necessary to identify genotype-trait associations. Many such associations have been detected, but it is not always clear which gene or other feature near the identified genomic location is functionally responsible for the association. Since these traits are in part mediated by gene expression, mapping the associations between genotype and gene expression in these animals will enable the discovery and deeper understanding of these trait associations. We therefore obtained genotypes and RNA-Seq gene expression for five brain regions from 88 HS rats and mapped expression quantitative trait loci (eQTLs) for each region. We identified cis-eQTLs in over 3,000 genes per brain region and validated their effect sizes using allele specific expression. This resource will enable new discoveries of the genetic influences of complex behavioral traits.
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| |
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| Overall design |
mRNA profiles from 4-5 brain regions from each of 88 naïve HS rats
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| |
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| Contributor(s) |
Munro D, Wang T, Chitre AS, Polesskaya O, Saba L, Chen H, Mohammadi P, Palmer AA |
| Citation(s) |
36263809 |
| |
| Submission date |
Apr 22, 2021 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Daniel Munro |
| E-mail(s) |
dmunro@scripps.edu
|
| Organization name |
UC San Diego
|
| Department |
Psychiatry
|
| Lab |
Palmer Lab
|
| Street address |
9500 Gilman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL22396 |
Illumina HiSeq 4000 (Rattus norvegicus) |
|
| Samples (86)
|
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| This SubSeries is part of SuperSeries: |
| GSE173141 |
Mapping genotype-expression associations in Heterogeneous Stock rat brains to advance behavioral genetics research |
|
| Relations |
| BioProject |
PRJNA723870 |
| SRA |
SRP315928 |
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