|
| Status |
Public on Sep 01, 2009 |
| Title |
GRE specific DNA-binding polyamide effect on dexamethasone-stimulated lung adenocarcinoma cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of dexamethasone-stimulated A549 lung adenocarcinoma epithelial cells treated with a glucocorticoid response (GR) element (GRE) specific DNA binding polyamide. Polyamide designed to target the sequence 5'-WGWWCW-3' and disrupt GR-mediated gene expression. Effects of the GR antagonist mifepristone also examined.
|
| |
|
| Overall design |
A549 cells were treated with compounds for 48 hours before RNA extraction and hybridization on Affymetrix microarrays.
|
| |
|
| Contributor(s) |
Muzikar KA, Nickols NG, Dervan PB |
| Citation(s) |
19805343 |
| |
| Submission date |
Jul 24, 2009 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Peter Dervan |
| Organization name |
California Institute of Technology
|
| Street address |
1200 East California Blvd
|
| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (15)
|
| GSM433026 |
A549 6 hours dexamethasone (1) |
| GSM433027 |
A549 6 hours dexamethasone (2) |
| GSM433028 |
A549 6 hours dexamethasone (3) |
| GSM433029 |
A549 48 hours mifepristone 6 hours dexamethasone (1) |
| GSM433030 |
A549 48 hours mifepristone 6 hours dexamethasone (2) |
| GSM433031 |
A549 48 hours mifepristone 6 hours dexamethasone (3) |
| GSM433032 |
A549 48 hours match polyamide 6 hours dexamethasone (1) |
| GSM433033 |
A549 48 hours match polyamide 6 hours dexamethasone (2) |
| GSM433034 |
A549 48 hours match polyamide 6 hours dexamethasone (3) |
| GSM433035 |
A549 48 hours mismatch polyamide 6 hours dexamethasone (1) |
| GSM433036 |
A549 48 hours mismatch polyamide 6 hours dexamethasone (2) |
| GSM433037 |
A549 48 hours mismatch polyamide 6 hours dexamethasone (3) |
|
| Relations |
| BioProject |
PRJNA118971 |
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