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| Status |
Public on Apr 29, 2022 |
| Title |
RNA sequencing of E15.5 retinal tissue from control and Lhx2 CKO mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Lhx2 is a retinal progenitor cell transcription factor critical for eye development. We previously reported that conditional inactivation of Lhx2 at the start of mouse retinal neurogenesis disrupted retinal progenitor cell (RPC) proliferation, greatly reduced the RPC pool and altered neurogenic output as indicated by changes in the production of multiple fated precursor populations. To identify genes whose expression levels are dependent on Lhx2 at this stage of development, Lhx2 conditional inactivation was initiated at E11.5 in RPCs with the progenitor Cre driver Hes1CreERT2 and retinal tissue was collected at E15.5 for RNA sequencing. The gene expression profiles of Lhx2 CKO retinas were compared to control (Lhx2 conditional heterozygotes) were compared. Downregulated and upregulated gene expression was observed, with some likely due to direct and indirect regulation by Lhx2 within RPCs and others due to changes in differentiation and the altered neurogenic output.
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| Overall design |
Lhx2 was inactivated by inducible Cre recombination using CreERT2 knocked into the Hes1 locus. Mice were heterozygous for Hes1CreERT2. Control mice were Lhx2flox/+ and experimental (CKO)mice were Lhx2flox/KO. Tamoxifen was gavage fed at E11.5 and embryos were harvested at E15.5. Each biological replicate contained both retinas from an individual embryo with 4 biological replicates for each condition (genotype).
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| Contributor(s) |
Levine EM, Gordon PJ |
| Citation(s) |
36459481 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 EY013760 |
Vsx2 Dependent Regulation of Retinal Progenitor Cell Properties |
VANDERBILT UNIVERSITY MEDICAL CENTER |
EDWARD M LEVINE |
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| Submission date |
Apr 20, 2021 |
| Last update date |
Dec 15, 2022 |
| Contact name |
EDWARD M LEVINE |
| E-mail(s) |
ed.levine@vanderbilt.edu, ed.levine@vumc.org
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| Organization name |
Vanderbilt University Medical Center
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| Department |
Vanderbilt Eye Institute
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| Street address |
1161 21st Ave S
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| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA723339 |
| SRA |
SRP315597 |
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