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Status |
Public on Jul 22, 2009 |
Title |
Transcriptome analysis of iron and phosphorus interaction in rice seedlings |
Organism |
Oryza sativa |
Experiment type |
Expression profiling by array
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Summary |
The antagonistic interaction between iron (Fe) and phosphorus (P) has been noted in the area of plant nutrition. To understand the physiology and molecular mechanisms of this interaction, we studied the growth performance, nutrient concentration, and gene expression profiles of root and shoot segments derived from 10-d-old rice (Oryza sativa) seedlings under four different nutrient conditions: (1) full strength of Fe and P (+Fe+P); (2) full strength of P and no Fe (-Fe+P); (3) full strength of Fe and no P (+Fe-P); and (4) without both Fe and P (-Fe-P). We used Affymetrix genechip to detail the global programme of gene expression underlying different Fe and P supply conditions identified distinct classes of up-regulated genes.
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Overall design |
The GeneChip analysis experiment was designed as a two-factor experiment with four treatments: (1) +P+Fe; (2) –Fe+P; (3) +Fe–P; (4) –Fe–P. The experiment included two biological replicates. Each sample represented root and shoot material from five to 10 plants.
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Contributor(s) |
Zheng L, Huang F, Narsai R, Wu J, Giraud E, He F, Cheng L, Wang F, Wu P, Whelan J, Shou H |
Citation(s) |
19605549 |
Submission date |
Jul 21, 2009 |
Last update date |
Dec 27, 2017 |
Contact name |
Luqing Zheng |
E-mail(s) |
zluqing@gmail.com
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Organization name |
Colledge of Life Sciences, Zhejiang University
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Street address |
388 Yuhangtanghe Road
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City |
Hangzhou |
ZIP/Postal code |
310058 |
Country |
China |
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Platforms (1) |
GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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Samples (16)
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Relations |
BioProject |
PRJNA118951 |