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| Status |
Public on Apr 19, 2024 |
| Title |
Loss of retinoic acid signaling drives immune escape in IDH mutant gliomas |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Gliomas are immunologically cold tumors that can be broken into several categories based on either RNA expression profiles or methylation profiles, with isocitrate dehydrogenase (IDH) mutations defining a major segregration between types. IDH mutant gliomas often exhibit defects in the retinoic acid pathway. We treated mice harboring IDH mutant gliomas with all-trans retinoic acid, and found that this treatment cause reductions in tumor growth and a swith in immune profiles in the tumor microenvironment.
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| Overall design |
Neurospheres (50,000 cells) were injected into the striatium of 6 week old mice. Mice were treated with either 10 mg/kg ATRA or control intra-peritoneally every other day until they were sacrificed based on signs of CNS pathology. 5-prime based single-cell RNAseq was then performed on manually dissected and digested tumors.
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| Contributor(s) |
Cillo AR, Rao A, Zhang X, Bruno TC, Vignali DA, Amankulor N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 20, 2021 |
| Last update date |
Apr 19, 2024 |
| Contact name |
Nduka Amankulor |
| E-mail(s) |
amankulornm@upmc.edu
|
| Organization name |
University of Pittsburgh
|
| Department |
Neurological Surgery
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| Street address |
200 Lothrop Street
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA723194 |
| SRA |
SRP315506 |
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