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Series GSE171845 Query DataSets for GSE171845
Status Public on Oct 01, 2021
Title Transcriptional adaptation to DNA replication stress induced by CCNE1 overexpression
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We overproduced human cyclin E from a stably-integrated inducible CCNE1 transgene in RPE1-hTERT cells over a period of weeks. CCNE1 overproduction induced replication stress, then slow proliferation, followed by adaptation to normal growth phenotype. We analyzed mRNA transcripts at key points of the timecourse: control, 2 days, 15-18 days, 39-51 days, and at a point several weeks after induction was withdrawn
 
Overall design Retinal pigmented epithelial (RPE1-hTERT) mRNA profiles of CCNE1 overexpression in adaptation experiment over period of up to 51 days
 
Contributor(s) Limas JC, Littlejohn AN, House AM, Kedziora KM, Aleman MM, Dominguez D, Cook JG
Citation(s) 35173014
Submission date Apr 11, 2021
Last update date Mar 15, 2022
Contact name Jeanette Cook
E-mail(s) jean_cook@med.unc.edu
Phone 9198433867
Organization name University of North Carolina at Chapel Hill
Department Biochemistry and Biophysics
Street address 112 Founders Ridge Dr.
City Chapel Hill
State/province North Carolina
ZIP/Postal code 27517
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (15)
GSM5235639 Control/Baseline population: 0 ng dox (no CCNE1 overexpression) [t0_1]
GSM5235640 Control/Baseline population: 0 ng dox (no CCNE1 overexpression) [t0_2]
GSM5235641 Control/Baseline population: 0 ng dox (no CCNE1 overexpression) [t0_3]
Relations
BioProject PRJNA721187
SRA SRP314351

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Supplementary file Size Download File type/resource
GSE171845_Limas2_all_TPM_table_ens_210121.xlsx 12.5 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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