|
| Status |
Public on Jun 28, 2021 |
| Title |
Bulk RNA-seq of fetal and adult murine Leydig cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To compare expression profiles of fetal and adult mouse Leydig cells, we performed bulk RNA sequencing.
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| |
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| Overall design |
Mouse testis from XY Nr5a1 Tg mouse embryos at embryonic day (E) 18.5 and 100-day old mice were harvested. Tissues were enzymatically dissociated and then filtered.The single cell solution is then sorted by fluorescent-active cell sorting to obtain a solution of alive and single Nr5a1-GFP+ cells. RNA was extracted from these cells with RNeasy Mini kit (Qiagen). Sequencing libraries were prepared with the TruSeq Stranded Total RNA Library Prep Gold (Ribo-Zero) and sequenced on an Illumina HiSeq 2500 (50 bp, paired end, ~35 million reads expected). Each condition was performed in three independent replicates.
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| Web link |
https://doi.org/10.3389/fcell.2021.695546
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| |
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| Contributor(s) |
Sararols P, Stévant I, Neirijnck Y, Nef S |
| Citation(s) |
34262907 |
| |
| Submission date |
Apr 08, 2021 |
| Last update date |
Jul 20, 2021 |
| Contact name |
Serge Nef |
| E-mail(s) |
serge.nef@unige.ch
|
| Organization name |
University of Geneva
|
| Department |
Genetic Medicine and Development
|
| Street address |
Rue Michel-Servet 1
|
| City |
Genève |
| ZIP/Postal code |
1206 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA720700 |
| SRA |
SRP314106 |
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