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| Status |
Public on Nov 15, 2021 |
| Title |
ADNP suppresses R-loops at its binding sites |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
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| Summary |
We have identified ADNP as an R-loop regulator using a proximity-dependent labeling system. We find that ADNP is necessary to suppress R-loops at its binding sites, and that deletion of the ADNP homeodomain reduces ADNP binding to chromatin and results in R-loop accumulation.
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| Overall design |
CUT&RUN, MapR, and RNA-Seq was performed on E14 mouse embryonic stem cells and human induced pluripotent stem cells. MNase-normalized tracks were generated for MapR samples by subtracting GST-MNase signal (negative control) from GST-RHΔ-Mnase signal.
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| Contributor(s) |
Wulfridge P, Yan Q, Doherty J, Fernandez-Luna JL, Real PJ, Tang HY, Sarma K |
| Citation(s) |
35013239 |
| |
| Submission date |
Apr 02, 2021 |
| Last update date |
Jan 28, 2022 |
| Contact name |
Phillip Wulfridge |
| E-mail(s) |
pwulfridge@wistar.org
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| Organization name |
The Wistar Institute
|
| Lab |
Sarma Lab
|
| Street address |
3601 Spruce St
|
| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA719393 |
| SRA |
SRP313259 |
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