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Series GSE16974 Query DataSets for GSE16974
Status Public on Nov 30, 2009
Title Retinal gene expression in Egr-1 knock-out mice during development (p30 and p42)
Organism Mus musculus
Experiment type Expression profiling by array
Summary In chicks, the avian homologue of the early growth response protein-1 (ZENK) has been shown to be increased in a special cell type of the retina, the glucagonergic amacrine cells, under conditions that lead to a reduction in eye growth (myopic defocus, recovery of myopia) and decreased under conditions that enhance ocular growth (hyperopic defocus, form-deprivation). The investigation of Egr-1 knock-out mice showed that homozygous knock-out mice with no functional Egr-1 protein developed relative axial myopia at the age of 42 and 56 days, compared to heterozygous- and wildtype Egr-1 knock-out mice.
To clarify the role of Egr-1 in the retinal regulation of eye growth, and to get an idea about the biochemical pathways underlying this mechanism, we studied the role of Egr-1 in more detail using Affymetrix microarrays.
Overall design Retinal samples of young homozygous Egr-1 knock-out and wildtype mice at the age of 30 days (hm30 and wt30; no difference in axial eye length yet) and 42 days (hm42 and wt42; already a difference in axial eye length of 59 ┬Ám) were taken to compare the mRNA expression changes over time between these two genotypes and within the same genotype between the two age groups.
Contributor(s) Schippert R, Schaeffel F, Feldkaemper MP
Citation(s) 20019881
Submission date Jul 07, 2009
Last update date Feb 11, 2019
Contact name Marita Pauline Feldkaemper
Phone +49-7071-2987424
Organization name University Eye Hospital Tuebingen, Institute for Ophthalmic Research
Department Section of Neurobiology of the Eye
Lab Prof. Dr. Frank Schaeffel
Street address Calwerstrasse 7/1
City Tuebingen
ZIP/Postal code 72074
Country Germany
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (16)
GSM424879 Retina wildtype 30 days 1
GSM424880 Retina wildtype 30 days 2
GSM424881 Retina wildtype 30 days 3
BioProject PRJNA117269

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16974_RAW.tar 54.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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