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Status |
Public on Sep 22, 2021 |
Title |
Transcriptome analysis of human pancreatic preadipocytes and in vitro differentiated adipocytes |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The aim of this study is to investigate the impact of the metabolic status on the transcriptome of isolated preadipocytes and in vitro differentiated adipocytes. We identified 38654 transcripts in pancreatic fat cells. We report that preadipocyte differentiation increased the abundance of mRNA levels of proteins related to adipogenesis and lipid metabolism. These changes in the transcriptome were absent or less pronounced in fat cells obtained from patients with prediabetes and type 2 diabetes. Meanwhile, mRNA levels of proteins involved in excellular matrix remodeling, angiogenesis and cytoskeleton organization were less abundant in adipocytes of all metabolic groups.
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Overall design |
Isolated preadipocytes and respective in vitro differentiated adipocytes from 12 different human donors stratified between non-diabetic, prediabetic and type 2 diabetic metabolic status.
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Contributor(s) |
Ullrich S, Oquendo MB, Dahl A, Czemmel S, Gauder M |
Citation(s) |
33788629 |
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Submission date |
Mar 24, 2021 |
Last update date |
Sep 23, 2021 |
Contact name |
Stefan Czemmel |
Organization name |
Quantitative Biology Center (QBiC)
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Department |
University of Tuebingen
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Street address |
Auf der Morgenstelle 10
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City |
Tuebingen |
ZIP/Postal code |
72076 |
Country |
Germany |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (24)
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Relations |
BioProject |
PRJNA716957 |
SRA |
SRP311952 |