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Series GSE169514 Query DataSets for GSE169514
Status Public on Sep 22, 2021
Title Transcriptome analysis of human pancreatic preadipocytes and in vitro differentiated adipocytes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The aim of this study is to investigate the impact of the metabolic status on the transcriptome of isolated preadipocytes and in vitro differentiated adipocytes. We identified 38654 transcripts in pancreatic fat cells. We report that preadipocyte differentiation increased the abundance of mRNA levels of proteins related to adipogenesis and lipid metabolism. These changes in the transcriptome were absent or less pronounced in fat cells obtained from patients with prediabetes and type 2 diabetes. Meanwhile, mRNA levels of proteins involved in excellular matrix remodeling, angiogenesis and cytoskeleton organization were less abundant in adipocytes of all metabolic groups.
 
Overall design Isolated preadipocytes and respective in vitro differentiated adipocytes from 12 different human donors stratified between non-diabetic, prediabetic and type 2 diabetic metabolic status.
 
Contributor(s) Ullrich S, Oquendo MB, Dahl A, Czemmel S, Gauder M
Citation(s) 33788629
Submission date Mar 24, 2021
Last update date Sep 23, 2021
Contact name Stefan Czemmel
Organization name Quantitative Biology Center (QBiC)
Department University of Tuebingen
Street address Auf der Morgenstelle 10
City Tuebingen
ZIP/Postal code 72076
Country Germany
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (24)
GSM5208123 Preadipocyte 1
GSM5208124 Preadipocyte 2
GSM5208125 Preadipocyte 3
Relations
BioProject PRJNA716957
SRA SRP311952

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Supplementary file Size Download File type/resource
GSE169514_merged_gene_counts_update.txt.gz 1.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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