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Series GSE168968 Query DataSets for GSE168968
Status Public on May 01, 2021
Title A CTP-dependent gating mechanism enables ParB spreading on DNA
Organisms Escherichia coli; Caulobacter vibrioides
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Proper chromosome segregation is essential in all living organisms. The ParA-ParB-parS system is widely employed for chromosome segregation in bacteria. Previously, we showed that Caulobacter crescentus ParB requires cytidine triphosphate to escape the nucleation site parS to spread by sliding to the neighboring DNA. Here, we provide the structural basis for this transition from nucleation to spreading by solving co-crystal structures of a C-terminal domain truncated C. crescentus ParB with parS and with a CTP analog. Nucleating ParB is an open clamp, in which parS is captured at the DNA-binding domain (the DNA-gate). Upon binding CTP, the N-terminal domain (NTD) self-dimerizes to close the NTD-gate of the clamp. The DNA-gate also closes, thus driving parS into a compartment between the DNA-gate and the C-terminal domain. CTP hydrolysis and/or the release of hydrolytic products may re-open the gates. Overall, we suggest a CTP-operated gating mechanism that regulates ParB nucleation and spreading.
Overall design Chromatin-immunoprecipitation with deep sequencing experiments (ChIP-seq) were performed on exponential-growing Caulobacter crescentus (see the supplementary Materials and Methods for the exact treatment that were applied to each strain).
Contributor(s) Jalal AS, Tran NT, Stevenson CE, Lawson DM, Le TB
Citation(s) 34397383
Submission date Mar 15, 2021
Last update date Sep 05, 2021
Contact name Tung Ba Khanh Le
Phone 01603450776
Organization name John Innes Centre
Department Department of Molecular Microbiology
Street address Colney Lane
City Norwich
State/province Norfolk
ZIP/Postal code NR4 7UH
Country United Kingdom
Platforms (2)
GPL18006 Illumina HiSeq 2500 (Caulobacter vibrioides)
GPL18133 Illumina HiSeq 2500 (Escherichia coli)
Samples (8)
GSM5173186 LELab ChIP seq TLS3050 anti FLAG replicate1
GSM5173187 LELab ChIP seq TLS3050 anti FLAG replicate2
GSM5173188 LELab ChIP seq TLS3059 anti FLAG replicate1
BioProject PRJNA714673
SRA SRP310754

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Supplementary file Size Download File type/resource
GSE168968_RAW.tar 107.3 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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