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Series GSE16868 Query DataSets for GSE16868
Status Public on Jan 28, 2013
Title Gene Expression between two cell layers in maize shoot apical meristem
Organism Zea mays
Experiment type Expression profiling by high throughput sequencing
Summary All above ground organs of higher plants are ultimately derived from shoot apical meristems (SAMs). The SAM exhibits distinctive structural organization, and monocot SAMs such as maize are comprised of two cell layers, a single cell layered tunica (L1) and a corpus (L2). Although recent research has revealed roles of these cell layers in the SAM, intra- and inter-cell-layer signaling networks involved in organ development remain largely unknown except for a few differentially expressed genes. Here, we used Illumnia technology to conduct RNA-seq of L1 and L2 cell layers in maize B73 maize shoot apical meristem.
 
Overall design Single sequencing library was constructed for L1 and L2 cell layer. Each library was sequenced using 2 lanes on a Solexa flow cell.
Processed data file 'ZmB73_4a.53_filtered_genes.fasta' and its README file are linked below as supplementary files. The fasta file contains the gene model ID and corresponding sequence generated from maize genome project. This fasta file was used for the following samples: GSM418173, GSM418174, GSM420173, GSM420174, GSM422828, GSM422829.
 
Contributor(s) Jia Y, Ohtsu K, Schnable PS
Citation(s) 19965430
Submission date Jun 27, 2009
Last update date May 23, 2013
Contact name Patrick S. Schnable
E-mail(s) schnable@iastate.edu
Phone 515-294-0975
Organization name Iowa State University
Street address 2035B Roy J Carver Co-Lab
City Ames
State/province IA
ZIP/Postal code 50011
Country USA
 
Platforms (1)
GPL9141 Illumina Genome Analyzer (Zea mays)
Samples (2)
GSM422828 Maize-SAM-L1
GSM422829 Maize-SAM-L2
Relations
SRA SRP001844
BioProject PRJNA117571

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16868_ZmB73_4a.53_filtered_genes.fasta.txt.gz 37.3 Mb (ftp)(http) TXT
GSE16868_filtered_genes_README.txt 572 b (ftp)(http) TXT
Processed data are available on Series record
Processed data provided as supplementary file
Processed data included within Sample table
Raw data are available in SRA

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