|
| Status |
Public on Apr 22, 2022 |
| Title |
Liver iNKT transcriptional profiling |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Invariant NKT (iNKT) cells comprise a heterogeneous group of terminally differentiated, non-circulating, tissue-resident T-lymphocytes that recognize glycolipids, including alpha-galactosylceramide (aGalCer), in the context of CD1d. Here, we show that murine and human liver-resident aGalCer/CD1d-binding iNKTs correspond to a novel Zbtb16+/Tbx21+/Gata3+/Maflow/Rorc– subset that exhibits profound plasticity. Repetitive encounters of these cells with intravenously-delivered aGalCer/CD1d-coated nanoparticles (NPs) trigger their differentiation into immunoregulatory, IL-10/IL-21-producing, Mafhigh cells expressing a T-regulatory type 1 (TR1)-like transcriptional signature.
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| Overall design |
We defined the transcriptional profile of iNKT cells from liver of untreated NOD.c3c4, NOD and B6 mice. We compared the transcriptional profile of aGalCer/CD1d-NP-treated and untreated NOD.c3c4 mice. Finally, we also characterized the transcriptional profile of human liver iNKT cells.
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| Contributor(s) |
Santamaria Vilanova P, Solé Sánchez P |
| Citation(s) |
35672409, 39315110 |
| |
| Submission date |
Mar 08, 2021 |
| Last update date |
Oct 02, 2024 |
| Contact name |
Pere Santamaria |
| E-mail(s) |
pesantam@clinic.cat
|
| Organization name |
IDIBAPS
|
| Street address |
C/Rosselló 149-153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platforms (3) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (19)
|
|
| Relations |
| BioProject |
PRJNA707550 |
| SRA |
SRP309811 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE168488_Human_aggr_barcodes.tsv.gz |
27.5 Kb |
(ftp)(http) |
TSV |
| GSE168488_Human_aggr_features.tsv.gz |
325.6 Kb |
(ftp)(http) |
TSV |
| GSE168488_Human_aggr_matrix.mtx.gz |
17.4 Mb |
(ftp)(http) |
MTX |
| GSE168488_Mouse_aggr_barcodes.tsv.gz |
83.8 Kb |
(ftp)(http) |
TSV |
| GSE168488_Mouse_aggr_features.tsv.gz |
272.8 Kb |
(ftp)(http) |
TSV |
| GSE168488_Mouse_aggr_matrix.mtx.gz |
68.2 Mb |
(ftp)(http) |
MTX |
| GSE168488_Normalized_counts_iNKT_c3c4_NOD_B6.xlsx |
2.6 Mb |
(ftp)(http) |
XLSX |
| GSE168488_Normalized_counts_iNKTs_Treated_Untreated.xlsx |
2.0 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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