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| Status |
Public on Mar 05, 2021 |
| Title |
Single cell RNA-seq analysis of bronchial epithelium in patients with severe SARS-CoV-2 pneumonia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To test whether airway epithelial and immune cells in patients with severe SARS-CoV-2 pneumonia exhibit specific gene expression signatures (namely interferon-response signature) we have performed bronchoscopy on intubated patients with severe SARS-CoV-2 pneumonia and obtained bronchial brushings.
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| Overall design |
Bronchial brushings from 4 patients with COVID-19 were obtained at Northwestern Memorial ICU. Single-cell libraries were prepared with 10x 5' V1 gene expression kit. Libraries were sequenced on Illumina NovaSeq 6000 and MiniSeq. Gene expression matrices were generated with 10x cellranger v3.1.0 software.
***RNA-Seq raw data is to be made available through dbGaP (controlled access) due to patient privacy concerns***
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| Contributor(s) |
Misharin A, Lu Z, Markov N, Wunderink R |
| Citation(s) |
34937051 |
| |
| Submission date |
Mar 04, 2021 |
| Last update date |
Feb 10, 2022 |
| Contact name |
Alexander V Misharin |
| E-mail(s) |
a-misharin@northwestern.edu
|
| Organization name |
Northwestern University
|
| Street address |
303 E Superior
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (2) |
| GPL27644 |
Illumina Novaseq 6000 (Homo sapiens) |
| GPL29811 |
Illumina Miniseq (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA706570 |
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