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Series GSE168137 Query DataSets for GSE168137
Status Public on Mar 04, 2021
Title Systematic Phenotyping and Characterization of the 5xFAD mouse model of Alzheimer’s Disease
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary we conducted a detailed phenotypic characterization of the 5xFAD model on a congenic C57BL/6J strain background, across its lifespan – including a seldomly analyzed 18-month old time point to provide temporally correlated phenotyping of this model and a template for characterization of new models of LOAD as they are generated. This comprehensive analysis included quantification of plaque burden, Aβ biochemical levels, and neuropathology, neurophysiological measurements and behavioral and cognitive assessments, and evaluation of microglia, astrocytes, and neurons. Analysis of transcriptional changes was conducted using bulk-tissue generated RNA-seq data from microdissected cortices and hippocampi as a function of aging, which can be explored at the UCI Mouse Explorer and AD Knowledge Portal. This deep-phenotyping pipeline identified novel aspects of age-related pathology in the 5xFAD model.
Overall design Bulk RNA-seq of 4 different time point(4,8,12,18 month) in two brain regoins (hippocampus and cortex) and two strains (5xFAD and BL6)
Contributor(s) Rezaie N, Mortazavi A
Citation(s) 34654824
NIH grant(s)
Grant ID Grant title Affiliation Name
U54 AG054349 UC Irvine AD Translational Center for Disease Model Resources The Regents of the University of California FRANK M LAFERLA
Submission date Mar 03, 2021
Last update date Nov 03, 2021
Contact name Narges Rezaie
Organization name University of California, Irvine
Street address 122 Verano place
City Irvine
State/province California
ZIP/Postal code 92617
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (192)
GSM5129591 5xFAD_cortex_4mon_female_430
GSM5129592 5xFAD_cortex_4mon_female_431
GSM5129593 5xFAD_cortex_4mon_female_433
BioProject PRJNA706199
SRA SRP309056

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Supplementary file Size Download File type/resource
GSE168137_countList.txt.gz 8.6 Mb (ftp)(http) TXT
GSE168137_expressionList.txt.gz 9.1 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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