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| Status |
Public on Oct 28, 2010 |
| Title |
Analysis of transcriptome in ectopic versus orthotopic thyroid tissue. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Congenital hypothyroidism from thyroid dysgenesis (CHTD) is a sporadic disease characterized by defects in the differentiation, migration or growth of thyroid tissue. Of these defects, incomplete migration resulting in ectopic thyroid tissue is the most common (up to 80%). We obtained flashfrozen samples of ectopic thyroid tissue removed from 3 girls aged 8, 10 and 15 yr, because it caused local symptoms. For comparison, we used orthotopic thyroid tissue from a Caucasian female, age 68 with gall bladder cancer. Analysis of transcriptome revealed up to 1011 genes more than twofold induced or repressed.
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| Overall design |
Microarray hybridization was performed on three independent pairs of ectopic thyroids (one pair performed in duplicate) compared to control thyroid tissue. After amplification and labelling, sample pairs were hybridized onto Human Exonic Evidence Based Oligonucleotide HEEBO slides (Stanford Functional Genomics Facility, CA). The oligonucleotide set consists of 44544 70-mer probes that were designed using a transcriptome-based annotation of exonic structure for genomic loci. Hybridizations were replicated with dye swap.
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| Contributor(s) |
Abu-Khudir R, Paquette J, Lefort A, Libert F, Chanoine J, Vassart G, Van Vliet G, Deladoëy J |
| Citation(s) |
20976176 |
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| Submission date |
Jun 25, 2009 |
| Last update date |
Aug 28, 2014 |
| Contact name |
Frederick Libert |
| E-mail(s) |
flibert@ulb.ac.be
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| Organization name |
ULB
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| Department |
IRIBHM
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| Lab |
C2-140
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| Street address |
808 route de Lennik
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| City |
Brussels |
| ZIP/Postal code |
B-1070 |
| Country |
Belgium |
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| Platforms (1) |
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| Samples (8)
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| GSM422030 |
HC22 versus normal Thyroid, biological replica 1_1 |
| GSM422031 |
HC22 versus normal Thyroid, biological replica 1_2 |
| GSM422032 |
HC23 versus normal Thyroid, biological replica 2_1 |
| GSM422033 |
HC23 versus normal Thyroid, biological replica 2_2 |
| GSM422034 |
HC6 versus normal Thyroid, biological replica 3_1 |
| GSM422035 |
HC6 versus normal Thyroid, biological replica 3_2 |
| GSM422036 |
HC6 versus normal Thyroid, biological replica 3_3 |
| GSM422037 |
HC6 versus normal Thyroid, biological replica 3_4 |
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| Relations |
| BioProject |
PRJNA117519 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16804_RAW.tar |
26.5 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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