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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 20, 2022 |
| Title |
Helicobacter pylori shows tropism to gastric differentiated pit cells dependent on urea chemotaxis. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The human gastric epithelium forms highly organized gland structures with different subtypes of cells. The carcinogenic bacterium Helicobacter pylori can attach to gastric cells and subsequently translocate its virulence factor CagA, but the possible host cell tropism of H. pylori is currently unknown. Here, we report that H. pylori preferentially attaches to differentiated cells in the pit region of gastric units. Single-cell RNA-seq shows that organoid-derived monolayers recapitulate the pit region, while organoids capture the gland region of the gastric units. Using these models, we show that H. pylori preferentially attaches to highly differentiated pit cells, marked by high levels of GKN1, GKN2 and PSCA. Directed differentiation of host cells enable enrichment of the target cell population and confirm H. pylori preferential attachment and CagA translocation into these cells. Attachment is independent of MUC5AC or PSCA expression, and instead relies on bacterial TlpB-dependent chemotaxis towards host cell-released urea, which scales with host cell size.
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| Overall design |
mRNA profiles from ex vivo human gastric units, 3D human gastric organoids, and 2D human gastric organoid-derived monolayers (naïve, bystander and H. pylori infected).
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| Contributor(s) |
Aguilar C, Pauzuolis M, Pompaiah M, Vafadarnejad E, Arampatzi P, Fischer M, Narres D, Neyazi M, Kayisoglu Ö, Sell T, Blüthgen N, Morkel M, Wiegering A, Germer C, Kircher S, Rosenwald A, Saliba A, Bartfeld S |
| Citation(s) |
36198679 |
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| Submission date |
Feb 25, 2021 |
| Last update date |
Oct 27, 2022 |
| Contact name |
Antoine-Emmanuel Saliba |
| E-mail(s) |
emmanuel.saliba@helmholtz-hzi.de
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| Phone |
+49-931-31-81341
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| Organization name |
Helmholtz Institute for RNA-based Infection Research
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| Street address |
Josef-Schneider-Straße 2 / D15
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| City |
Würzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
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| Platforms (3) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (5)
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| GSM5107550 |
Gastric organoid-derived monolayer bystander (C9) |
| GSM5107551 |
Gastric organoid-derived monolayer infected (C10) |
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| Relations |
| BioProject |
PRJNA704962 |
| SRA |
SRP308239 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE167561_RAW.tar |
26.8 Mb |
(http)(custom) |
TAR (of TAR) |
| GSE167561_README_A1A3C8C9C10.txt |
314 b |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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