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| Status |
Public on Oct 05, 2021 |
| Title |
Bulk RNA-seq of 3D Cultured Intestinal Organoids (Ileum and Colon) from Satb2-deficient and WT mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The colonic microenvironment may have influenced gene expression of the ileal-like mucosa in Satb2KO colon In Vivo. We use 3D culture organoid system In Vitro to mitigate the environmental influence.
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| Overall design |
All organoids were estimated in lagre intestine culture medium and passaged one generation. The differentiated organoids were collected for Bulk RNA-Seq
Please note that the additional raw counts data (prior to normalization), which was generated from the process of generation of counts relative to genes using featureCounts with default parameters (unstranded mode), was included in the records on Nov 5, 2021.
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| |
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| Contributor(s) |
Gu W, Huang X, Zhou Q |
| Citation(s) |
34582804 |
| |
| Submission date |
Feb 22, 2021 |
| Last update date |
Nov 05, 2021 |
| Contact name |
Wei Gu |
| E-mail(s) |
wei.gu@beigene.com
|
| Phone |
13776921246
|
| Organization name |
Beigene
|
| Department |
Beigene Institute
|
| Lab |
Gu Lab
|
| Street address |
51 Rijing Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200135 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE148695 |
SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling |
|
| Relations |
| BioProject |
PRJNA704070 |
| SRA |
SRP307573 |
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