|
| Status |
Public on Oct 20, 2021 |
| Title |
Single cell RNA sequencing of myeloid cells from lungs of young and aged mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single cell RNA sequencing from lungs of young (2-3 month old) and aged (20-22 month old) mice.
|
| |
|
| Overall design |
Myeloid cells (CD45+ Gr1+ CD11b+) were sorted from lungs of young and aged mice that were perfused. Cells from young mice (2-3 months old) were labeled with TotalSeq-C0951 SA antibody (Barcode sequence AACCTTTGCCACTGC), and cells from aged mice (20-22 months old) were labeled with TotalSeq-C0952 SA antibody (Barcode sequence GTCCGACTAATAGCT). Cells were then pooled for scRNAseq preparation.
|
| |
|
| Contributor(s) |
D'Souza S, Yang Q |
| Citation(s) |
34547174 |
| |
| Submission date |
Feb 22, 2021 |
| Last update date |
Jan 21, 2022 |
| Contact name |
Qi Yang |
| E-mail(s) |
qy117@rwjms.rutgers.edu
|
| Organization name |
Rutgers RWJMS
|
| Street address |
89 French St
|
| City |
New Brunswick |
| State/province |
NJ |
| ZIP/Postal code |
08901 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (1) |
| GSM5100147 |
Myeloid cells from young and aged mouse lungs |
|
| Relations |
| BioProject |
PRJNA703968 |
| SRA |
SRP307524 |
