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| Status |
Public on Jan 31, 2024 |
| Title |
The complex role of Naked cuticle in Drosophila wing development |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
It is of tremendous interests to understand how Wnt signaling is regulated in a precise manner both temporally and spatially. Naked cuticle (Nkd) acts as a negative-feedback inhibitor for Wingless (Wg, a fly Wnt) signaling in Drosophila embryonic development. In the present study, we show that nkd is essential for wing pattern formation, such that both gain and loss of nkd result in the disruption of Wg target expression in larvae stage and abnormal adult wing morphologies. Our results of high-throughput sequencing suggest that nkd have profound effects on Drosophila wing development with several critical signaling pathways involved, we conclude that Nkd inhibits the canonical Wg pathway during Drosophila wing development beyond embryonic stage and may play a mediated role in cross-talk between development-related pathways.
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| Overall design |
mRNA profiles of third instar larval wing discs expressing GFP or Nkd recombinant proteins under the control of MS1096-Gal4.
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| Contributor(s) |
Wang R, Xie H, Yang L, Wang P |
| Citation missing |
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| |
| Submission date |
Feb 20, 2021 |
| Last update date |
Jan 31, 2024 |
| Contact name |
Rui Wang |
| Organization name |
Southeast University
|
| Department |
School of Life Science and Technology
|
| Lab |
MOE Key Laboratory of Developmental Genes and Human Diseases
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| Street address |
2 Sipailou Road
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210096 |
| Country |
China |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA703379 |
| SRA |
SRP307239 |
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