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| Status |
Public on Feb 01, 2022 |
| Title |
Interaction between PPARγ and mTORC1 in the regulation of inguinal white adipose tissue transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To investigate the involvement of mTORC1 as a mediator of the actions of the PPARγ ligand rosiglitazone in subcutaneous inguinal white adipose tissue transcriptome; Methods: Mice bearing regulatory associated protein of mTOR (Raptor) deletion and therefore mTORC1 deficiency exclusively in adipocytes (adiponectin Cre recombinase) and littermate controls were fed a high-fat diet supplemented or not with the PPARγ agonist rosiglitazone (30 mg/kg/day) for 8 weeks and evaluated for inguinal white adipose tissue transcriptome (Rnaseq); Results: 3,2425 genes had their correspondent mRNA levels altered by either adipocyte Raptor deficiency or rosiglitazone administration or their combination. Among those, 408 genes modulated by rosiglitazone required mTORC1. Conclusion: PPARγ and mTORC1 are essential partners in the regulation of a cluster of genes in inguinal white adipose tissue.
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| Overall design |
Mice bearing regulatory associated protein of mTOR (Raptor) deletion and therefore mTORC1 deficiency exclusively in adipocytes (adiponectin Cre recombinase) and littermate controls were fed a high-fat diet supplemented or not with the PPARγ agonist rosiglitazone (30 mg/kg/day) for 8 weeks and evaluated for inguinal white adipose tissue transcriptome (Rnaseq)
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| Contributor(s) |
Andrade ML, Festuccia WT |
| Citation(s) |
34004356 |
| |
| Submission date |
Feb 17, 2021 |
| Last update date |
Aug 26, 2022 |
| Contact name |
NORC Boston |
| E-mail(s) |
fungen.core@gmail.com
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| Organization name |
Beth Israel Deaconess Medical Center
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| Street address |
3 Blackfan Circle, CLS0733
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (11)
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| GSM5090405 |
Inguinal white adipose tissue Raptor KO + rosiglitazone Replicate 1 |
| GSM5090406 |
Inguinal white adipose tissue Raptor KO + rosiglitazone Replicate 2 |
| GSM5090407 |
Inguinal white adipose tissue Raptor KO + rosiglitazone Replicate 3 |
| GSM5090408 |
Inguinal white adipose tissue Raptor KO Replicate 1 |
| GSM5090409 |
Inguinal white adipose tissue Raptor KO Replicate 2 |
| GSM5090410 |
Inguinal white adipose tissue Raptor WT Replicate 1 |
| GSM5090411 |
Inguinal white adipose tissue Raptor WT Replicate 2 |
| GSM5090412 |
Inguinal white adipose tissue Raptor WT Replicate 3 |
| GSM5090413 |
Inguinal white adipose tissue Raptor WT + rosiglitazone Replicate 1 |
| GSM5090414 |
Inguinal white adipose tissue Raptor WT + rosiglitazone Replicate 2 |
| GSM5090415 |
Inguinal white adipose tissue Raptor WT + rosiglitazone Replicate 3 |
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| Relations |
| BioProject |
PRJNA702528 |
| SRA |
SRP306898 |
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