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Series GSE166609 Query DataSets for GSE166609
Status Public on Mar 26, 2021
Title The AXL-PYK2-PKCα axis as a nexus of stemness circuits in TNBC
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Cancer stem cells (CSCs) are implicated in tumor initiation, metastasis and drug resistance, and are considered as attractive targets for cancer therapy. We identified a clinically relevant signaling nexus mediated by PYK2 and its impact on stemness in triple negative breast cancer (TNBC). PYK2 depletion in multiple mesenchymal TNBC cell lines markedly reduced the expression of PKCα and AXL proteins and reduced the number of mammosphere-forming cells and cells harboring CSCs characteristic markers. PYK2 and PKCα cooperate at a convergence point of multiple stemness-inducing pathways to regulate AXL levels and concomitantly the levels/activation of key transcription factors implicated in stemness including STAT3, TAZ, FRA1 and SMAD3 as well as the pluripotent transcription factors Nanog and Oct4, and in-turn affect their target genes. Targeting the AXL-PYK2-PKCα circuit could be a promising strategy to eliminate CSCs in TNBC and possibly overcome drug resistance.
Overall design PYK2 (PTK2B) was knocked-down in the triple negative breast cancer cell line MDA-MB-231 using shRNA. The empty lentiviral vector (PLKO) was used as control. Experiment was done in duplicates.
Contributor(s) Lev S, Khera L, Vinik Y
Citation(s) 33785524
Submission date Feb 11, 2021
Last update date Apr 05, 2021
Contact name Sima Lev
Organization name Weizmann Institute of Science
Department Molecular Cell Biology
Street address 234 Herzl St.
City Rehovot
ZIP/Postal code 7610001
Country Israel
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (4)
GSM5076183 MDA231_PLKO_a [57_Y]
GSM5076184 MDA231_shPYK2_a [58_Y]
GSM5076185 MDA231_PLKO_b [59_Y]
BioProject PRJNA701409
SRA SRP305833

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Supplementary file Size Download File type/resource
GSE166609_count_matrix.csv.gz 266.2 Kb (ftp)(http) CSV
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