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Series GSE16650 Query DataSets for GSE16650
Status Public on Aug 31, 2009
Title Expression data from Bronchial Epithelial Cells exposed to Cyclic Stretch with and without LPS or TNFa
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Acute respiratory distress syndrome (ARDS) is a catastrophic form of acute lung injury (ALI). The necessity for mechanical ventilation (MV) renders patients at risk for ventilator induced lung injury (VILI). Exposure to repetitive cyclic stretch (CS) and/or over-inflation exacerbates injury. Reducing tidal volume (VT) is the only therapeutic strategy shown to mitigate morbidity and mortality. Cyclic stretch has been shown to differentially regulate gene expression in part through the activation of mammalian mitogen-activated protein kinase (MAPK). Although these studies have shown both molecular and cellular alterations, no unifying hypothesis to explain MV-induced lung injury has emerged.
In the current study, we hypothesized that coordinated expression of cyclic stretch (CS)-responsive genes relies on the presence of common CS-sensitive regulatory elements. To identify CS-responsive genes, we undertook a comparative examination of the gene expression profile of human bronchial epithelial airway (Beas-2B) cells in response to various injurious stimuli involved in the pathogenesis of acute lung injury (ALI)/Ventilator induced lung injury (VILI): cyclic stretch, tumor necrosis factor alpha (TNF-a), and lipopolysaccharide (LPS).
Overall design Human Bronchial Epithelial Cells (Beas-B2) cells grown on silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S]. Total RNA (duplicate experiments) was extracted using TRIZOL reagent (as per manufactures specifications) and purified using Qiagen mRNA purification Kit (as per manufacturers specifications). mRNA was hybridized to Affymetrix Human U133plus2.0 chips. Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).
Contributor(s) Akram A, Han B, Masoom H, Peng C, Lam E, Litvak M, Bai X, Shan Y, Hai T, Slutsky AS, Zhang H, Haitsma JJ, Liu M, dos Santos CC
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Submission date Jun 16, 2009
Last update date Jan 08, 2019
Contact name Claudia Chimisso dos Santos
Organization name Saint Michaels Hospital
Department University of Toronto
Street address 30, Bond Street, room 4-011
City Toronto
State/province ON
ZIP/Postal code M5B 1W8
Country Canada
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (12)
GSM417770 Control I BEAS2b_ML01C
GSM417771 Stretch I BEAS2b_ML02C
GSM417772 LPS I BEAS2b_ML03C
BioProject PRJNA116237

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Supplementary file Size Download File type/resource
GSE16650_RAW.tar 63.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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