|
| Status |
Public on Jan 07, 2022 |
| Title |
Transcriptional repression and apoptosis influence the effect of APOBEC3A/3B functional polymorphisms on biliary tract cancer risk |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
APOBEC3s-related somatic mutations are the predominant burden in biliary tract cancers (BTCs). Here, we reveal the effects and mechanisms of APOBEC3A/3B functional polymorphisms on cholangiocarcinoma and gallbladder cancer (GBC). rs2267401-G at the APOBEC3B promoter decreases cholangiocarcinoma risk but increased GBC risk. rs2267401-G confers a decreased APOBEC3B promoter activity in cholangiocarcinoma cells but an increased activity in GBC cells. rs12157810-C at the APOBEC3A promoter decreases the risk of BTCs. rs12157810-C up-regulated the promoter activity in both cells. APOBEC3A overexpression attenuates cancer evolution via causing apoptosis, in contrast to APOBEC3B. Inflammatory factors promote cancer evolution via interacting with transcriptional repressors regulating the APOBEC3A/3B promoters. ATAC-seq was used to identify the difference between transcriptional networks of cholangiocarcinoma and GBC.
|
| |
|
| Overall design |
4 Cell lines were involved in the single-cell ATAC-seq analysis: the cholangiocarcinoma cell line RBE, the RBE with stimulation of TNF-a, GBC, and GBC with stimulation of TNF-a
|
| |
|
| Contributor(s) |
Liu W, Ji H, Song J, Cao G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 08, 2021 |
| Last update date |
Jan 10, 2022 |
| Contact name |
Wenbin Liu |
| E-mail(s) |
wenbinl_lxb@sina.com
|
| Phone |
+86-13127696716
|
| Organization name |
Second Military Medical University
|
| Street address |
800 Xiangyin Rd..
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA700633 |
| SRA |
SRP305336 |
Less...
More...