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| Status |
Public on Feb 05, 2021 |
| Title |
Bulk and single RNA sequencing on the role of Igf2bp2 in myeloid-biased HSC |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using RNA sequencing analysis on myeloid biased HSC, we compared gene expression profiles between WT and Igf2bp2 knock-out mice at young and old age. 1421 differentially expressed genes were identified in young myeloid biased HSC from Igf2bp2 knock-out mice compared to young WT; however, only 26 differentially expressed genes were identified in old myeloid biased HSC from Igf2bp2 knock-out mice compared to old WT. Compared to young WT myeloid biased HSC, myeloid biased HSC from young Igf2bp2 knock-out exhibits reduced expression of genes involved in metabolism and translation. In addition, single cell RNA sequencing analysis of myeloid biased HSC in young wildtype mice identified nine sub-clusters. HSCs from a sub-cluster with high expression of Igf2bp2 exhibit up-regulated IGF/PI3K/AKT signaling and increased expression of genes involved in HSC quiescence and maintenance. Altogether, the study provides a correlation and mechanism to understand the role of Igf2bp2 in HSC function and aging.
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| Overall design |
RNA sequencing of myeloid-biased HSC from Wild Type and Igf2bp2-/- mice at young and old; single cell RNA sequencing of myeloid-biased HSC from Wild Type at 6 week old
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| Contributor(s) |
Suo M, Bens M, Görlich I |
| Citation(s) |
35231105 |
| |
| Submission date |
Feb 04, 2021 |
| Last update date |
May 01, 2024 |
| Contact name |
Pura Muñoz-Cánoves |
| Organization name |
Altos Labs
|
| Lab |
Muñoz-Cánoves
|
| Street address |
5510 Morehouse Dr
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (17)
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| Relations |
| BioProject |
PRJNA699541 |
| SRA |
SRP304738 |
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