|
| Status |
Public on Sep 01, 2021 |
| Title |
Mechanistic dissection of dominant AIRE mutations in mouse models reveals AIRE auto-regulation [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Using mouse models of several recessive and putative dominant-negative patient mutations we were able to dissect the mechanisms that underlie the recessive and dominant-negative capacities of the corresponding AIRE mutants, including a newly identified dominant-negative variant C446G
We also identify an auto-regulatory mechanism by which AIRE negatively modulates its own expression.
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| |
|
| Overall design |
ChIPseq profiles of AIRE binding in sorted mature medullary thymic epithelial cells (mTEChi) from young (4-7 weeks old) Aire +/+, Aire +/C313Y and Aire +/C442G mice generated in the study.
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| |
|
| Contributor(s) |
Jakub A, Yael G |
| Citation(s) |
34477806 |
| |
| Submission date |
Jan 26, 2021 |
| Last update date |
Nov 29, 2021 |
| Contact name |
Jakub Abramson |
| E-mail(s) |
jakub.abramson@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Department of Immunology
|
| Street address |
234 Herzl st.
|
| City |
Rehovot |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE151013 |
Mechanistic dissection of dominant AIRE mutations in mouse models reveals evidence for AIRE auto-regulation |
|
| Relations |
| BioProject |
PRJNA694945 |
| SRA |
SRP303340 |
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