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Series GSE165409 Query DataSets for GSE165409
Status Public on Feb 09, 2021
Title Arterial stiffness and cardiac dysfunction in Hutchinson-Gilford Progeria Syndrome corrected by inhibition of Lysyl Oxidase
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Arterial stiffening is a hallmark of premature aging in Hutchinson-Gilford Progeria Syndrome (HGPS), but the molecular regulators remain unknown. Here, we show that the LMNAG609G mouse model of HGPS recapitulates the premature arterial stiffening seen in human HGPS. To gain a better understanding of potential stiffness-regulators in LMNAG609G mice, we performed RNA-sequencing analysis on cleaned descending aortas from 2- and 24-month WT and 2-month LMNAG609G mice on a C57BL6 background.
Methods: Descending aortas containing the intimal, medial and adventitial layers were isolated from 2- and 24-month male WT and 2-month HGPS mice, and RNA was extracted using the RNeasy Plus Micro kit (Qiagen 74034). The high-throughput library was prepared using the TruSeq stranded total RNA (ribo-Zero) kit (Illumina 20037135). Paired-end sequencing was performed on a HiSeq4000 Sequencing System (Illumina) and generated 14-30 million reads/sample.
 
Overall design Aortic mRNA profiles of young and old wild type (WT) and young LMNAG609G mice.
 
Contributor(s) Assoian RK, Tobias JW
Citation(s) 33687998
Submission date Jan 24, 2021
Last update date May 12, 2021
Contact name Ryan Von Kleeck
E-mail(s) ryanvon@pennmedicine.upenn.edu
Organization name University of Pennsylvania
Street address 421 Curie Blvd
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (18)
GSM5032948 LMNAG609G Rep 1
GSM5032949 LMNAG609G Rep 2
GSM5032950 LMNAG609G Rep 3
Relations
BioProject PRJNA694470
SRA SRP303066

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE165409_LMNA_rawCounts_18samp.txt.gz 748.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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