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| Status |
Public on Feb 27, 2022 |
| Title |
Dissecting the stem cell compartment in Csnk1a1 haploinsufficiency |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We sorted hematopoietic progenitors from mouse bone marrow previously transplanted with either Csnk1a1 fl/+ or Mx1Cre+ bone marrow and performed scRNAseq. We identified cell populations in a continuum of differentiation from quiescent stem cell to cycling lineage-marker expressing progenitor. Our analysis revealed signaling changes in primitive stem cells with Csnk1a1 haploinsufficiency that result in downregulation of inflammatory NFkB signaling while upregulating Myc and E2f dependent pro-proliferative pathways
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| Overall design |
We used scRNA 3' RNA sequencing product from 10x Genomics to investigate the stem cell compartment in murine models of Csnk1a1 haploinsufficiency
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| Contributor(s) |
Stalmann US, i Li R, Costa IG, Schneider RK |
| Citation(s) |
35016204 |
| Submission date |
Jan 24, 2021 |
| Last update date |
Mar 01, 2022 |
| Contact name |
Ronghui Li |
| E-mail(s) |
hilironghui@gmail.com
|
| Organization name |
RWTH Aachen
|
| Lab |
nstitute for Computational Genomics
|
| Street address |
Pauwelsstr. 19
|
| City |
Aachen |
| ZIP/Postal code |
52074 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA694424 |
| SRA |
SRP303017 |
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