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| Status |
Public on May 16, 2021 |
| Title |
Transcriptional analysis of SARS-CoV-2 specific CD4 T cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The objective of this analysis was to determine if there was a transcriptional difference in the CD4 T cell response elicited by SARS-CoV-2 infection in individuals that quickly resolved their infection compared to those individuals with prolonged SARS-CoV-2-associated symptoms
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| |
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| Overall design |
A total of PBMC 10 samples from convalescent COVID-19 patients were stimulated in vitro with SARS-CoV-2 Spike protein peptide pools overnight. Spike-reactive CD4 T cells were identified by merit of their upregulation of CD69 and CD134 in response to peptide stimulation. Antigen-specific cells were sorted directly into RLT+ lysis buffer for transcriptional analysis
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| |
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| Contributor(s) |
Waickman A |
| Citation(s) |
34063463 |
| |
| Submission date |
Jan 22, 2021 |
| Last update date |
Aug 16, 2021 |
| Contact name |
Adam Tully Waickman |
| Organization name |
SUNY Upstate Medical University
|
| Street address |
766 Irving Avenue
|
| City |
Syracuse |
| State/province |
New York |
| ZIP/Postal code |
13210 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA694174 |
| SRA |
SRP302926 |
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