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| Status |
Public on Jun 01, 2023 |
| Title |
Genome-wide binding profile of Smad3 and Smad7 in mouse Ch12 cell line (RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mammalian genome encodes approximately 1,700 transcription factors (TFs), 1,300 out of which have sequence specific binding motifs. Transcription in mammalian cells is regulated by the recruitment of TFs to specific cis-regulatory elements. In spite of consistent efforts on the function of individual TF, the question still remains how TFs bind to DNA and form enhancer. Here, we try to solve this problem by investigating the relationship between TF binding pattern and chromatin accessibility (ATAC-Seq). We first systematically acquired ATAC-Seq dataset as well as matched RNA-Seq dataset from different mouse primary tissues. A comprehensive TF binding map was built for each tissue/cell type by genomic approaches.
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| Overall design |
We analyzed genome-wide binding profile of Smad3 and Smad7 in mouse Ch12 cell line.
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| Contributor(s) |
Zhao Y, Conte A, Vartak S, Casellas R |
| Citation missing |
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| Submission date |
Jan 22, 2021 |
| Last update date |
Jun 03, 2023 |
| Contact name |
Yongbing Zhao |
| Phone |
3014805852
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| Organization name |
National Institutes of Health
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| Department |
NIAMS
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| Lab |
Casellas Lab
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| Street address |
10 Center Drive RooM 13C101A
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| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA694115 |
| SRA |
SRP302880 |
