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Series GSE16533 Query DataSets for GSE16533
Status Public on Jun 10, 2010
Title Expression data from lenses triply deficient for E2F1, E2F2, and E2F3 transcription factors
Organism Mus musculus
Experiment type Expression profiling by array
Summary The E2F family consists of transcriptional repressors and activators that control cell proliferation. In the classic paradigm of cell cycle regulation, the three activators, E2F1, E2F2 and E2F3, are invariably depicted as the final components of a CDK/Rb signaling cascade that executes the transcriptional program necessary to commit cells to enter S phase.
Unexpectedly, we find through analysis of Affymetrix expression array data that mature lens epithelial cells deficient for E2F1-3 fail to repress cell cycle-regulated genes (and other targets of E2F) and that this corresponds with subsequent apoptosis and cellular collapse in the lens.
Overall design Murine lenses were collected at two stages of development for RNA extraction and hybridization on Affymetrix microarrays. Our aim was to determine key events that lead to cellular collapse of lenses triply deficient for E2F1, E2F2, and E2F3 in neonates.
Contributor(s) Wenzel PL, Leone G
Citation(s) 21185283
Submission date Jun 10, 2009
Last update date Feb 11, 2019
Contact name Pamela L. Wenzel
Phone 617-919-2016
Fax 617-730-0222
Organization name Children's Hospital Boston
Department Hematology/Oncology
Lab George Daley
Street address 300 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (20)
GSM415205 E2F2-/- P0_01
GSM415206 E2F2-/- P0_05
GSM415207 E2F2-/- P0_06
BioProject PRJNA116103

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16533_RAW.tar 67.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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